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Full Description
Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more.
Contents
Section 1 Microfluidics for cell culture, cell manipulation and cell sorting1. Viable cell culture in PDMS-based microfluidic devicesMelikhan Tanyeri and Savas Tay2. High-throughput microfluidic single-cell trapping arrays for biomolecular and imaging analysisXuan Li and Abraham P. Lee3. Artificial niche microarrays for identifying extrinsic cell-fate determinantsSamy Gobaa, Raphael V. Gayet and Matthias P. Lutolf4. Reconstruction of directed neuronal networks in a microfluidic device with asymmetric microchannelsJosquin Courte, Renaud Renault, Audric Jan, Jean-Louis Viovy, Jean-Michel Peyrin and Catherine Villard5. Single-cell 3D electro-rotationLiang Huang, Peng Zhao, Fei Liang and Wenhui Wang
Section 2 Droplet-based microfluidics6. Direct quantification of EGFR variant allele frequency in cell-free DNA using a microfluidic-free digital droplet PCR assayBenjamin Demaree, Daniel Weisgerber, Ata Dolatmoradi, Makiko Hatori and Adam R. Abate7. Quantifying phenotypes in single cells using droplet microfluidicsFengjiao Lyu, Lucas R. Blauch and Sindy K.Y. Tang8. Modular microfluidics for double emulsion formationBryant Thompson, Nareh Movsesian, Christine Cheng, Prathamesh Karandikar, Malancha Gupta and Noah Malmstadt9. Universal anchored-droplet device for cellular bioassaysGabriel Amselem, Sébastien Sart and Charles N. Baroud
Section 3 Microfluidics for cellular analysis10. Cell biology at the interface of nanobiosensors and microfluidicsNikhil Bhalla, Hung-Ju Chiang and Amy Q. Shen11. BET-seq: Binding energy topographies revealed by microfluidics and high-throughput sequencingArjun K. Aditham, Tyler C. Shimko and Polly M. Fordyce
