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Full Description
Mitosis and Meiosis, Part B, Volume 145, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Mitotic live cell imaging at different time scales, the characterization of mitotic spindle by multi-mode correlative microscopy, STED microscopy of mitosis, Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture, quantification of three-dimensional spindle architecture, Imaging based assays for mitotic chromosome condensation and dynamics, and more.
Contents
Preface
Helder Maiato and Melina Schuh
1. Mitotic live-cell imaging at different timescales
Anna-Maria Olziersky, Chris A. Smith, Nigel Burroughs, Andrew D. McAinsh and Patrick Meraldi
2. Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture
Nicholas I. Clarke and Stephen J. Royle
3. Quantification of three-dimensional spindle architecture
Norbert Lindow, Stefanie Redemann, Florian Brünig, Gunar Fabig, Thomas Müller-Reichert and Steffen Prohaska
4. Quantitative live and super-resolution microscopy of mitotic chromosomes
Nike Walther and Jan Ellenberg
5. Live imaging of cell division in 3D stem-cell organoid cultures
Ana C.F. Bolhaqueiro, Richard H. van Jaarsveld, Bas Ponsioen, René M. Overmeer, Hugo J. Snippert and Geert J.P.L. Kops
6. Choosing the right microscope to image mitosis in zebrafish embryos: A practical guide
Iskra Yanakieva, Marija Matejcic and Caren Norden
7. Advanced microscopy methods for bioimaging of mitotic microtubules in plants
Petra Vyplelová, Miroslav Ovečka, George Komis and Jozef Šamaj
8. Microsurgery and microinjection techniques in mitosis research
Charles A. Day, Jessica Hornick, Alyssa Langfald, Christopher Mader and Edward H. Hinchcliffe
9. Analyzing the micromechanics of the cell division apparatus
Yuta Shimamoto and Tarun M. Kapoor
10. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells
Ana Milas, Mihaela Jagrić, Jelena Martinčić and Iva M. Tolić
11. Live imaging of C. elegans oocytes and early embryos
Kimberley Laband, Benjamin Lacroix, Frances Edwards, Julie C. Canman and Julien Dumont
12. Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes
Pierre Romé and Hiroyuki Ohkura
13. Methods to study meiosis in insect spermatocytes
James R. LaFountain, Jr and Christopher S. Cohan
14. Live-cell microscopy of meiosis in spermatocytes
Hiroki Shibuya and Yoshinori Watanabe
15. Live imaging of cell division in preimplantation mouse embryos using inverted light-sheet microscopy
Judith Reichmann, Manuel Eguren, Yu Lin, Isabell Schneider and Jan Ellenberg
16. Correlated light and electron microscopy of cell division in large marine oocytes, eggs, and embryos
Mariia Burdyniuk, Natalia Wesolowska, Michal Fleszar, Matthia A. Karreman, Pedro Machado, Joana Borrego-Pinto, Bernhard Ruthensteiner, Yannick Schwab and Péter Lénárt
17. A microscopy-based approach for studying meiosis in live and fixed human oocytes
Agata P. Zielinska and Melina Schuh
18. Superresolution imaging of the synaptonemal complex
Katharina Schücker, Markus Sauer and Ricardo Benavente
19. Cryoelectron tomography of SYCP3 fibres under native conditions
Daniel Bollschweiler, Laura Radu and Luca Pellegrini
