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Full Description
Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.
Contents
1. A High-Throughput Flurometric Assay for Lipid-Protein Binding
Wonhwa Cho
2. Fluorescence-based in situ quantitative imaging for cellular lipids
Wonhwa Cho
3. Recombinant Expression of human and mouse groups I, II, III, V, V and XII Secreted Phospholipases A2
Michael H. Gelb and Gerard Lambeau
4. Vesicle-based assay of secreted phospholipase A2 enzymatic activity for high throughput screening of compound libraries
Michael H. Gelb
5. Cellular Assays for Evaluating Calcium-Dependent Translocation of cPLA2a to Membrane
Christina C. Leslie
6. Secreted Phospholipase A2 Specificity on Natural Membrane Phospholipids
Makoto Murakami
7. Analyses of Calcium-Independent Phospholipase A2beta (iPLA2β) in Biological Systems
Sasanka Ramanadham
8. Using hydrogen deuterium exchange mass spectrometry to examine protein membrane interactions
John E. Burke
9. Lipid-binding C2 domains mediate a diverse class of enzymatic functions"
Robert V. Stahelin
10. Interfacial Enzymes: Membrane Binding, Orientation, Membrane Insertion, and Activity
Suren Tatulian
11. Enzymology at the Membrane Interface
Dan M. Raben
12. Studying gastric lipase adsorption onto phospholipid monolayers by surface tensiometry, ellipsometry and atomic force microscopy
Frédéric Carrière
13. Probing conformational changes and interfacial recognition site of lipases with surfactants and inhibitors
Frédéric Carrière
14. Measuring Phospholipase D enzymatic activity through biochemical and imaging methods
Michael A. Frohman
15. Pld Rotein-Protein Interaction with Intracellular Signaling Molecules, Modulation by Mitogen Pa and Pld's Gef Activity
Julian G. Cambronero
16. Phosphatidic Acid Regulation of PIPKI
Guangwei Du