Full Description
Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data.
This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science.
Contents
1. The Polymerase Chain Reaction: PCR, qPCR, and RT-PCR
2. Methods of Cloning
3. Whole-Mount In Situ Hybridization and a Genotyping Method on Single Xenopus Embryos
4. Microarrays: An Introduction and Guide to Their Use
5. Analysis of Human Genetic Variations Using DNA Sequencing
6. Western Blot
7. The Enzyme-Linked Immunosorbent Assay: The Application of ELISA in Clinical Research
8. Immunofluorescence
9. Cell Culture: Growing Cells as Model Systems In Vitro
10. Flow Cytometry
11. Transfection
12. In Vivo Animal Modeling: Drosophila
13. Zebrafish as a Research Organism: Danio rerio in Biomedical Research
14. Xenopus as a Model Organism for Biomedical Research
15. Basic Mouse Methods for Clinician Researchers: Harnessing the Mouse for Biomedical Research
