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Full Description
The cell interior is another world that we are only beginning to explore. Although there are a number of approaches for examining the inner workings of the cell, the reductionist approach of building up complexity appeals to many with physical science and engineering backgrounds. This volume of Methods in Cell Biology spans a range of spatial scales from single protein molecules to vesicle and cell sized structures capable of complex behaviors. Contributions include; methods for combining different motors and cytoskeletal components in defined ways to produce more complex behaviors; methods to combine cytoskeletal assemblies with fabricated devices such as chambers or pillar arrays; reconstituting membrane fission and fusion; reconstituting important biological processes that normally take place on membrane surfaces; and methods for encapsulating protein machines within vesicles or droplets.
Contents
1. In Vitro Systems for the Study of Microtubule-Based Cell Polarity in Fission YeastNúria Taberner, Andries Lof, Sophie Roth, Dimitry Lamers, Hans Zeijlemaker and Marileen Dogterom2. Microtubules, MAPs, and Motor PatternsKasimira T. Stanhope and Jennifer L. Ross3. Self-Organization of Motors and Microtubules in Lipid-Monolayered DropletsHella Baumann and Thomas Surrey4. Reconstitution of Microtubule Based Motility Using Cell ExtractsSwathi Ayloo and Erika L. F. Holzbaur5. Building Cells for Quantitative, Live-cell Analyses of Collective Motor Protein FunctionsEric A. Kumar, David Tsao, Anand Radhakrishnan and Michael Diehl6. Reconstituting Cytoskeletal Contraction Events with Biomimetic Actin-Myosin Active gelsJosé Alvarado and Gijsje H. Koenderink7. Building an Artificial Actin Cortex on Microscopic Pillar ArraysR. Ayadi and W. H. Roos8. Triggering Actin Polymerization in Xenopus Egg Extracts from Phosphoinositide-Containing Lipid BilayersAstrid Walrant, Daniel S. Saxton, Guilherme Pereira Correia and Jennifer L. Gallop9. Reconstituting Geometry Modulated Protein Patterns in Membrane CompartmentsKatja Zieske and Petra Schwille10. Structural and Functional Studies of Membrane Remodeling MachinesRaghav Kalia, Nathaniel Talledge and Adam Frost11. Building Interconnected Membrane NetworksMatthew A. Holden12. Using Supported Bilayers to Study the Spatiotemporal Organization of Membrane Bound ProteinsPhuong A. Nguyen, Christine M. Field, Aaron C. Groen, Timothy J. Mitchison and Martin Loose 13. Reconstituting ParA/ParB-Mediated Transport of DNA CargoAnthony G. Vecchiarelli, James A. Taylor and Kiyoshi Mizuuchi14. Cell-Sized Liposomes that Mimic Cell Motility and the Cell CortexJoël Lemière, Kevin Carvalho and Cécile Sykes15. Reconstitution of Cortical Actin Networks Within Water-In-Oil EmulsionsEnas Abu Shah, Maya Malik-Garbi and Kinneret Keren16. Engineering Artificial Cells by Combining HeLa-Based Cell-Free Expression and Ultra-Thin Double Emulsion TemplateKenneth K.Y. Ho, Victoria L. Murray, Allen P. Liu17. Reconstitution of Proteins on Electroformed Giant Unilamellar VesiclesEva M. Schmid, David L. Richmond, and Daniel A. Fletcher18. Reconstituting SNARE-Mediated Membrane Fusion at the Single Liposome LevelVolker Kiessling, Binyong Liang and Lukas K. Tamm
