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Full Description
Laboratory Techniques in Rabies Diagnosis, Research and Prevention provides a basic understanding of the current trends in rabies. It establishes a new facility for rabies surveillance, vaccine and antibody manufacturing. It offers clarity about the choice of laboratory methods for diagnosis and virus typing, of systems for producing monoclonal and polyclonal antibodies and of methods for testing potency of vaccines and antibodies.The book covers advancements in the classical methods described as well as recent methods and approaches pertaining to rabies diagnosis and research.
Contents
I. Foreword
II. Introduction
III. Rabies Diagnosis
a) Demonstration of virus
i) Electron microscopy
ii) Virus isolation in animals, the mouse inoculation test (MIT)
iii) Virus isolation in cell culture, the rapid tissue culture inoculation test (RTCIT)
iv) Rat basophilic leukemia cell sensor
b) Demonstration of viral subunits and antigens
i) Purification of rabies viral subunits
ii) Preparation of fluorescent antibody conjugate in rabbits
iii) Preparation of fluorescent antibody conjugate in goats
iv) DFA (postmortem)
v) Skin biopsy
vi) Immuno histochemistry
c) Demonstration of viral nucleic acids
i) Gel based PCR
ii) In situ PCR
d) Demonstration of viral antibodies
1. Binding Assays
i) ELISA - Traditional
ii) IFA
iii) Counter Immunoelectrophoresis
2. Functional Assays
i) Mouse neutralization test
ii) RFFIT
iii) FAVN
e) Typing/differentiation of lyssaviruses
i) Antigenic typing by MAbs
IV. Rabies Biologics
i) Rabies vaccines for humans or other animals
1. Purified chick embryo cell vaccine
2. Purified Vero Cell Rabies Vaccine
3. In vivo NIH potency testing of rabies vaccines
9. In vitro Potency testing of rabies vaccines
ii) Rabies antibodies/fragments
1. Production of polyclonal rabies immune globulin in humans
2. Production of polyclonal antibodies in horses
3. Production of polyclonal antibodies in birds
4. Production of full length human monoclonal antibodies using transgenic mice
V. Development of Anti-Viral Approaches
VI. Appendix
i. List of biologics recommended by WHO for human rabies prophylaxis
