Full Description
Epigenetic Biomarkers and Diagnostics comprises 31 chapters contributed by leading active researchers in basic and clinical epigenetics. The book begins with the basis of epigenetic mechanisms and descriptions of epigenetic biomarkers that can be used in clinical diagnostics and prognostics. It goes on to discuss classical methods and next generation sequencing-based technologies to discover and analyze epigenetic biomarkers. The book concludes with an account of DNA methylation, post-translational modifications and noncoding RNAs as the most promising biomarkers for cancer (i.e. breast, lung, colon, etc.), metabolic disorders (i.e. diabetes and obesity), autoimmune diseases, infertility, allergy, infectious diseases, and neurological disorders.
The book describes the challenging aspects of research in epigenetics, and current findings regarding new epigenetic elements and modifiers, providing guidance for researchers interested in the most advanced technologies and tested biomarkers to be used in the clinical diagnosis or prognosis of disease.
Contents
Preface
José Luis García-Giménez
1. Epigenetic Biomarkers: New findings, perspectives, and future directions in diagnostics
José Luis García-Giménez, Toshikazu Ushijima, and Trygve O. Tollefsbol
2. The importance of Biobanks in epigenetic studies
Lorena Peiró-Chova, Olga Bahamonde Ponce, Carolina Abril-Tormo, Jacobo Martínez-Santamaría, José Antonio López-Guerrero and Peter H.J. Riegman
3. Epigenetic Mechanisms as Key Regulators in Disease. Clinical Implications
Abdelhalim Boukaba, José Luis García-Giménez
4. The evolution of new technologies and methods in clinical epigenetics research
Tandy L. Dolin Petrov and Nicole C. Riddle
5. Biomarkers and methodologies for monitoring epigenetic drug effects in cancer
Marianne B. Treppendahl, Lasse Sommer Kristensen and Kirsten Grønbæk
6. Genome-wide techniques for the study of clinical epigenetic biomarkers
Eneda Toska and F Javier Carmona Sanz
7. Sequenom MassARRAY technology for the analysis of DNA methylation. Clinical applications
Enrique J. Buso and Marisa Iborra
8. The role of Methylation-specific PCR and associated techniques in clinical diagnostics
Fang Zhao and Bharati Bapat
9. Pyrosequencing and its application in epigenetic clinical diagnostics
Ana-Maria Florea
10. Mass spectrometry for the identification of post-translational modifications in histones and its application in clinical epigenetics
Roberta Noberini, Alessandro Cuomo and Tiziana Bonaldi
11. High-throughput analysis of non-coding RNAs: implications in clinical epigenetics
Valerio Costa, Maria R Matarazzo, Miriam Gagliardi, Roberta Esposito, Alfredo Ciccodicola
12. Circulating non-coding RNAs as clinical biomarkers
Francesco Russo, Flavia Scoyni, Alessandro Fatica, Marco Pellegrini, Alfredo Ferro, Alfredo Pulvirenti and Rosalba Giugno
13. DNA methylation biomarkers in lung cancer
Juan Sandoval and Paula Lopez Serra
14. DNA methylation alterations as biomarkers for Prostate Cancer
João Ramalho-Carvalho, Rui Henrique, Carmen Jerónimo
15. DNA methylation in breast cancer
Raimundo Cervera, Alberto Ramos, Ana Lluch and Joan Climent
16. DNA methylation in obesity and associated diseases
Ana B Crujeiras and Angel Díaz-Lagares
17. DNA Methylation Biomarkers in Asthma and Allergy
Avery DeVries and Donata Vercelli
18. DNA Methylation in Pregnancy Complications
Deepali Sundrani, Vinita Khot and Sadhana Joshi
19. Epigenetics in Infectious Diseases
Genevieve Syn, Jenefer M. Blackwell and Sarra E. Jamieson
20. DNA Methylation in Neurodegenerative Diseases
Sahar Al-Mahdawi, Sara Anjomani Virmouni and Mark A. Pook
21. The histone code and disease: post-translational modifications as potential prognostic factors for clinical diagnosis
Nicolas G. Simonet, George Rasti and Alejandro Vaquero
22. Histone Post-Translational Modifications and Chromatin Remodelers in Prostate Cancer
Filipa Quintela Vieira, Diogo Almeida-Rios, Inês Graça, Rui Henrique and Carmen Jerónimo
23. Histone post-translational modifications in breast cancer and their use in clinical diagnosis and prognosis
Luca Magnani, Annita Louloupi and Wilbert Zwart
24. Histone variants and post-translational modifications in spermatogenesis and infertility
Juan Ausio, Yinan Zhang and Toyotaka Ishibashi
25. Circulating histones and nucleosomes as biomarkers in sepsis and septic shock
José Luis García Giménez, Carlos Romá Mateo, Marta Seco Cervera, José Santiago Ibañez Cabellos and Federico V. Pallardó
26. Chromatin landscape and epigenetic signatures in neurological disorders: emerging perspectives for biological and clinical research
Pamela Milani and Ernest Fraenkel
27. MicroRNAs deregulation in lung cancer and their use as clinical tools
Paula Lopez-Serra and Juan Sandoval
28. Circulating Nucleic Acids as Prostate Cancer Biomarkers
Claire E. Fletcher, Ailsa Sita-Lumsden, Akifumi Shibakawa and Charlotte L. Bevan
29. microRNAs in breast cancer and their value as biomarkers
Olafur Andri Stefansson
30. microRNAs in bone and soft tissue sarcomas and their value as biomarkers
Tomohiro Fujiwara, Yu Fujita, Yutaka Nezu, Akira Kawai, Toshifumi Ozaki, Takahiro Ochiya
31. miRNAs in the pathophysiology of diabetes and their value as biomarkers
Eoin Brennan, Aaron McClelland, Shinji Hagiwara, Catherine Godson and Phillip Kantharidis
