Full Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods providing a a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo. Malignant cells exhibit metabolic changes when compared to their normal counterparts, owing to both genetic and epigenetic alterations. Although such a metabolic rewiring has recently been indicated as "yet another" general hallmark of cancer, accumulating evidence suggests that the metabolic alterations of each neoplasm rather represent a molecular signature that intimately accompanies, and hence cannot be severed from, all facets of malignant transformation.
Contents
1. Methods to measure cytoplasmic and mitochondrial Ca2+ concentration using Ca2+-sensitive dyes2. Methods to measure intracellular Ca2+ fluxes with Organelle-Targeted aequorin-based probes3. Methods to measure baseline Ca2+ levels with second-generation organelle-targeted fluorescent probes4. Methods to assess the autophagic flux in malignant cells5. Methods to assess autophagy in situ-transmission electron microscopy versus immunohistochemistry6. Methods to measure the enzymatic activity of PI3Ks7. Luciferase-based reporter to monitor the transcriptional activity of the SIRT3 promoter8. Metabolomic profiling of cultured cancer cells9. Pulsed stable isotope-resolved metabolomic studies of cancer cells10. Single-cell imaging for the study of oncometabolism 11. Study of cellular oncometabolism via multi-dimensional protein identification technology12. In vivo quantitative proteomics for the study of oncometabolism13. Metabolomic profiling of neoplastic lesions in mice14. Metabolomic profiling of tumor-bearing mice15. Metabolomic studies of patient material by high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy16. Analysis of metabolomic profiling data acquired on GC-MS
