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Full Description
This new volume of Methods in Cell Biology looks at micropatterning in cell biology and includes chapters on protein photo-patterning on PEG with benzophenone, laser-directed cell printing and dip pen nanolithography. The cutting-edge material in this comprehensive collection is intended to guide researchers for years to come.
Contents
Section 1 Micropatterning soft substrates
1 Preparation of a Micropatterned Rigid-Soft Composite Substrate for Probing Cellular Rigidity Sensing
2 Patterned Hydrogels for Simplified Measurement of Cell Traction Forces
3 Micropatterning Hydroxy-PAAm Hydrogels and Sylgard 184 Silicone Elastomers with Tunable Elastic Moduli
4 The Facile Generation of 2-dimensional Stiffness Maps in Durotactic Cell Platforms Through Thickness Projections of 3-dimensional Submerged Topography
5 Micropatterning on Micropost Arrays
6 Development of Micropatterned Cell-Sensing Surfaces
7 Microfluidic Patterning of Protein Gradients on Biomimetic Hydrogel Substrates
Section 2 Cell and tissue micropatterning in 3D
8 Micropatterning of Poly(ethylene glycol) Diacrylate Hydrogels
9 Curved and Folded Micropatterns in 3D Cell Culture and Tissue Engineering
10 Cell Migration in Confined Environments
11 Micro-patterned Porous Membranes for Combinatorial Cell-based Assays
12 Micropatterning Cells on Permeable Membrane Filters
13 Microfabrication of a Platform to Measure and Manipulate the Mechanics of Engineered Microtissues
14 Methods for 2D Cell Confinement
15 Benzophenone-based Photochemical Micropatterning of Biomolecules to Create Model Substrates and Instructive Biomaterials
