Full Description
Regulated Cell Death Part A & Part B of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in apoptosis focusing on the important areas of intrinsic pathway, extrinsic pathway, caspases, cellular assays and post-apoptotic effects and model organisms; as well as topics on necroptosis and screening approaches.
Contents
1. Examining the Molecular Mechanism of Bcl-2 Family Proteins at Membranes by Fluorescence Spectroscopy.
2. Photoreactive Stapled Peptides to Identify and Characterize BCL-2 Family Interaction Sites by Mass Spectrometry
3. The Structural Biology of BH3-only proteins
4. How to Analyze Mitochondrial Morphology in Healthy Cells and Apoptotic Cells in Caenorhabditis elegans
5. Apoptosis Initiation Through the Cell-Extrinsic Pathway
6. Using RNAi Screening Technologies to Interrogate the Extrinsic Apoptosis Pathway
7. Caspase Enzymology and Activation Mechanisms
8. Turning ON Caspases with Genetics and Small Molecules
9. A Multi-Pronged Approach for Compiling a Global Map of Allosteric Regulation in the Apoptotic Caspases
10. Measuring Caspase Activity in vivo
11. Single-Molecule Sensing of Caspase Activation in Live Cells via Plasmon Coupling Nanotechnology
12. In vivo Monitoring of Caspase Activation Using a Fluorescence Resonance Energy Transfer-Based Tfluorescent Probe
13. Global Analysis of Cellular Proteolysis by Selective Enzymatic Labeling of Protein N-termini
14. Complementary Methods for the Identification of Substrates of Proteolysis
15. Phospholipid Scrambling on the Plasma Membrane
16. Studying Apoptosis in the Zebrafish
