Full Description
This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds
Contents
Simplified Synthetic Antibody Libraries
Generation of Dual Variable Domain Immunoglobulin Molecules for Dual-specific Targeting
Discovery of internalizing antibodies to tumor antigens from phage libraries
Optimizing properties of anti-receptor antibodies using kinetic computational models and experiments
Cell-specific siRNA delivery by peptides and antibodies
Conjugation of Anticancer Drugs Through Endogenous Monoclonal Antibody Cysteine Residues
Building and characterizing antibody-targeted lipidic nanotherapeutics
Cell-Targeting Fusion Constructs Containing Recombinant Gelonin
Stapled Peptides for Intracellular Drug Targets
Mapping of vascular zip codes by phage display
Engineering cyclic peptide toxins
Peptide Discovery using Bacterial Display and Flow Cytometry
Oral enzyme therapy for celiac sprue
Rational Design and Evaluation of Mammalian Ribonuclease Cytotoxins
Engineering Reduced-Immunogenicity Enzymes for Amino Acid Depletion Therapy in Cancer
Designed Ankyrin Repeat Proteins (DARPins): From research to therapy
Target-binding proteins based on the tenth human fibronectin type III domain (10Fn3)
Anticalins: small engineered binding proteins based on the lipocalin scaffold
T Cell Receptor Engineering
Engineering Knottins as Novel Binding Agents
Re-Engineering Biopharmaceuticals for Targeted Delivery Across the Blood-Brain Barrier
Practical Theoretic Guidance for the Design of Tumor Targeting Agents
Fusions of elastin-like polypeptides to pharmaceutical proteins
Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells
