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基本説明
Comprehensive guide to essential methods in cell biology.
Full Description
This text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems.
Contents
Permeabilized Epithelial Cells to Study Exocytic Membrane Transport
Studying Excit and Surface Delivery of Post-Golgi Transport Intermediates Using in vitro and Live-Cell Microscopy-Based Approaches
Assays Measuring Membrane Transport in the Endocytic Pathway
Microsome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport In Mammalian Cells
Cotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells
Use of Permeabilsed Mast Cells to Analyze Regulated Exocytosis
Syringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell Loading
Cell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial Monolayers
Protein Translocation into Mitochondria
Polarographic Assays of Mitochrondrial Functions
Analysis of Nuclear Protein Import and Export in Digitonin-Permeabilized Cells
Heterokaryons: An Assay for Nucleocytoplasmic Shuttling
DNA Replication-Dependent Chromatin Assembly System
Cygnets: Intracellular Guanosine 3',5-Cycli Monophosphate Sensing in Primary Cells Using Fluorescence Energy Transfer
Ca2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos)
Ratiometric Pericam
Fuorescent Indicators for Imaging Protein Phosphorylation in Single Living Cells
In Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32P Labeling of Cellular Proteins
Dissecting Pathways; in Situ Electroporation for the Study of Signal Transduction and Gap Junctional Communication
Detection of Protein-Protein Interactions in vivo Using Cyan and Yellow Fluorescent Proteins
Tracking Individual Chromosomes with Integrated Arrays of Iacop Sites and GFP-laci Repressor: Analyzing Position and Dynamics of Chromosomal Loci in Saccharomyces cerevisiae
Microtubule Motility Assays
In vitro Assays for Mitotic Spindle Assembly and Function
In vitro Motility Assays with Actin
Use of Brain Cytosolic Extracts for Studying Actin-Based Motility of Listeria monocytogenes
Pedestal Formation by Pathogenic Escherichia coli: A Model System for Studying Signal Transduction to the Actin Cytoskeleton
Listeria monocytogenes: Techniques to Analyze Bacterial Infection in vitro
Measurement of Cellular Contractile Forces Using Patterned Elastomer
