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基本説明
An updated edition of the classic Methods in Cell Biology volume 48, this book, along with its companion volume, emphasizes diverse methods and technologies needed to investigate C. elegans. Contents: Development, Neurobiology, and Behavior; Molecular Biology and Biochemistry; Cell Biology and Physiology.
Full Description
Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.
Contents
Recent advances in Cytometry Instrumentation, Probes and Methods
Anja Mittag, Arkadiusz Pierzchalski, and Attila Tárnok
Section I. Down-sizing cytometry to "micro" dimension
Droplet Microfluidics for High-throughput Analysis of Cells and Particles
Michele Zagnoni and Jonathan M. Cooper
Parallel Imaging Microfluidic Cytometer
Daniel J. Ehrlich, Brian K. McKenna, James G. Evans, Anna C. Belkina, Gerald V. Denis, David H. Sherr and Man Ching Cheung
Microfluidic Systems for Live Cell Imaging
Philip Lee, Terry Gaige and Paul Hung
Rise of the micro-machines: microfluidics and the future of cytometry
Donald Wlodkowic and Zbigniew Darzynkiewicz
Label-Free Resistive-Pulse Cytometry
M. R. Chapman and L. L. Sohn
Section II. Imaging cytometry
Laser Scanning Cytometry and Its Applications: A Pioneering Technology in the Field of Quantitative Imaging Cytometry
Melvin Henriksen, Bruce Miller, Judith Newmark, Yousef Al-Kofahi and Elena Holden
Analytical capabilities of ImageStream cytometer
Ewa K. Zuba-Surma and Mariusz Z. Ratajczak
Laser scanning cytometry: capturing the immune system in situ
Mairi A McGrath, Angela M Morton and Margaret M Harnett
Image cytometry analysis of circulating tumor cells
Lori E. Lowes, David Goodale, Michael Keeney and Alison L. Allan
Pre-clinical Applications of Quantitative Imaging Cytometry to Support Drug Discovery
David L Krull and Richard A Peterson
Leveraging Image Cytometry for the Development of Clinically Feasible Biomarkers: Evaluation of Activated Caspase-3 in Fine Needle Aspirate Biopsies
Gloria Juan, Stephen J. Zoog and John Ferbas
Automation of the buccal micronucleus cytome assay using laser scanning cytometry
Wayne R. Leifert, Maxime François, Philip Thomas, Ed Luther, Elena Holden and Michael Fenech
Laser Scanning Cytometry of Mitosis:state and stage analysis
Tammy Stefan and James W. Jacobberger
Section III. Instrumentation, new probes and methods
Lasers in Flow Cytometry
William G. Telford
The use of hollow fiber membranes combined with cytometry in analysis of bacteriological samples
Kawiak J, Stachowiak R, Lyżniak M, Bielecki J, Granicka L
Guide to red fluorescent proteins and biosensors for flow cytometry
Kiryl D. Piatkevich and Vladislav V. Verkhusha
Quantum Dot Technology in Flow Cytometry
Pratip K. Chattopadhyay
Background-free Cytometry Using Rare Earth Complex Bioprobes
Dayong Jin
Surface Enhanced Raman Scattering (SERS) Cytometry
John P. Nolan and David S. Sebba
Recent Advances in Flow Cytometric Cell Sorting
Geoffrey W. Osborne
