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基本説明
Dedicated to disseminating information on methods in determining the biological roles of carbohydrates.
Full Description
In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the second of three in the Methods in Enzymology series, including Glycobiology (vol. 415) and Glycomics (vol. 416), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses.
Contents
Section I. N-Glycan Function Revealed by Gene Inactivation
Cytokine Sensitivity and N-Glycan Processing Mutations
Phenotype Changes of Fut8 Knockout Mouse: Core Fucosylation Is Crucial for the Function of Growth Factor Receptor(s)
Section II. Neural Cell Function
Cellular and Molecular Analysis of Neural Development of Glycosyltransferase Gene Knockout Mice
Roles of Glycolipids in the Development and Maintenance of Nervous Tissues
Analysis of Neural Cell Functions in Gene Knockout Mice: Electrophysiological Investigation of Synaptic Plasticity in Acute Hippocampal Slices
Analysis of Neural Cell Function in Gene Knockout Mice: Behavior
Electrophysiological Analysis of Interactions Between Carbohydrates and Transmitter Receptors Reconstituted in Lipid Bilayers
Section III. Notch Signaling and Muscular Dystrophy
Methods for Analysis of O-Linked Modifications on Epidermal Growth Factor-Like and Thrombospondin Type 1 Repeats
Roles of O-Fucosyltransferase 1 and O-Linked Fucose in Notch Receptor Function
Roles of O-Fucose Glycans in Notch Signaling Revealed by Mutant Mice
Defect in Glycosylation that Causes Muscular Dystrophy
Section IV. Glycolipid Function
Identification and Analysis of Novel Glycolipids in Vertebrate Brains by HPLC/Mass Spectrometry
Modulation of Growth Factor Signaling by Gangliosides: Positive Or Negative?
Activation of Natural Killer T Cells by Glycolipids
Section V. Glycolipid Interaction
Determination of Glycolipid-Protein Interaction Specificity
Analysis of Carbohydrate-Carbohydrate Interactions Using Gold Glyconanoparticles and Oligosaccharide Self-Assembling Monolayers
Section VI. Galectin Function
Galectin Interactions with Extracellular Matrix and Effects on Cellular Function
Galectins in Apoptosis
On the Role of Galectins in Signal Transduction
Section VII. Newly Developed Field
Helicobacter pylori Adhesion to Carbohydrates
Caenorhabditis elegans Carbohydrates in Bacterial Toxin Resistance
N-Glycans Are Involved in the Response of Caenorhabditis elegans to Bacterial Pathogens
Detection of Cytoplasmic Glycosylation Associated with Hydroxyproline
Author Index
Subject Index
