北口哲也・鈴木団（共）編／細胞の可視化と操作のためのタンパク質工学 Engineering Proteins to Visualize and Manipulate Cells

著者名：Kitaguchi, Tetsuya (EDT)/Suzuki, Madoka (EDT)

Humana（2026/10/29発売）

• 言語：ENG
• ISBN：9781071656860
• eISBN：9781071656877

Description

This detailed volume highlights cutting-edge strategies and technologies that enable scientists to engineer proteins with the objective of visualizing and manipulating cells. From imaging intracellular molecules to manipulating cellular behavior with accuracy, this book explores how engineered proteins are redefining biological research, opening new horizons for understanding protein interactions, disease management, sustainability, and innovation, and what is possible for larger societal benefits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Engineering Proteins to Visualize and Manipulate Cells aims to stimulate new perspectives on cell visualization and manipulation and advance the boundaries of biological discovery.

Table of Contents

Highly-Biocompatible Multiplexed Super-Resolution Imaging by SPoD-OnSPAN.- Quantitative Imaging of Calcium Dynamics with a Green Fluorescent Biosensor and Fluorescence Lifetime Imaging.- Genetically Encoded Multimeric Nanoparticles for Measuring Intracellular Diffusion and Cytoplasmic Viscosity.- GPCR-Based Genetically Encoded Fluorescent Indicators.- Using All Probes Plasmids to Lower the Barrier for Quantifying Single-mRNA Translation Dynamics in Live Cells.- Full-Spectrum Spectroscopic Characterization of Extracellular pH Effects on FRET-Based Genetic Voltage Indicators Expressed in Xenopus laevis Oocytes.- Super Photostable Organic Dye for Single Molecule Imaging and Single Particle Tracking in Live Cells.- Optochemical Proximity Labeling for Mapping Endogenous Receptor Networks in the Live Mouse Brain.- Tailored Methods to Develop High-Performance Chemigenetic Indicators.- Cell Labeling with Fluorescent Nanodiamonds for Visualization and Manipulation.- Radiation-Crosslinked Protein Hydrogels and Nanogels: Preparation and Applications in Cell Culture and Nanomedicine.- Generating and Evaluating New Chemigenetic Fluorescent Biosensors for Protein Kinase A Based on Synthetic Organic Fluorophores.- Wash-Free Live-Cell Imaging Using Homogeneous Immunosensors.- Optogenetic Tool for Controlling p53 Signaling Pathway.- Chemo-Optogenetic Control of Protein Activities in Living Cells Using Photoswitchable Molecular Glues.- Reversible Sequestration and Release of GFP-Tagged Proteins in Mammalian Cells Using Small-Molecule-Responsive Synthetic Condensates.- Calibration of Intracellular Thermometers Using Localized Microheating.- Protocells with Fluorescent Response to Extracellular Signals.- An Effective Pipeline to Develop Modification-Specific Intracellular Antibodies Using AI Tools.- Engineered Antibody-Like Bivalent Binders for Manipulating Receptor Functions.- Using the Function Prediction Server and Queryome for Functional Protein Design.