Description
This third edition volume expands on the previous editions with an update on phospholipases, including structure-function relationships using the new tools of artificial intelligence, as well as current and new applications of both enzymes. The chapters in this book are organized into three parts: Part One provides readers with an overview on lipases and phospholipases; Part Two covers quantitative methods for assessing lipase activity and case studies on cloning and comparison of host for lipase expression; and Part Three presents relevant applications of lipases in new emerging fields such as nanobiocatalysis, polymers synthesis/degradation, and biosensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Lipases and Phospholipases: Methods and Protocols, Third Edition is a practical and detailed reference for both graduate students and expert scientists. This book will be useful to the scientific community working with lipases, phospholipases, and related enzymes.
Table of Contents
Lipases: An Overview.- Phospholipases: Where Do We Stand?.- Quantitative Methods for Assessing True Lipase Activity Using Long-Chain Triglycerides and Complex Lipid Substrates.- Cloning and Protein Expression of CpEst in Komagataella phaffii.- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.- Lipase Activity, Analytical Methods: Brief Review and Gold Standards.- Phospholipases D: An Update on Structure – Function Relationships.- Synthesis of Esters of 5-Hydroxymethylfurfural Catalyzed by Lipases in a Continuous Packed-Bed Bioreactor.- Synthesis and Purification of Lipase-Catalyzed Sugar E.- Lipase-Catalyzed Synthesis of Nutritional Structured Lipids at Laboratory-Scale Using Crude of Refined Oils.- Lipase-Catalyzed Pathways to the Synthesis of Pharmacologically Active Amides.- Lipases and Cutinases from Phytopathogenic and Saprobic Fungi in Plastic Depolymerization and Synthesis: A Review.- Biopolymers Derived from Solanum lycopersicum: Synthesis with CAL-B and Their Structural Analysis by Mass Spectrometry (DI-ESI and MALDI-TOF).- Functionalization of Flavonoids with Lipases.- Two-Step Lipase-Catalyzed Acylation of Non-Glycosylated Flavonoids: Application to Phloretin.- Assessment of the Hypoglycemic and Hypolipidemic Activities of Fructans and Their Laurate Bioconjugates.- Bio-Inspired Silica Lipase Nanobiocatalysts for the Synthesis of Fatty Acid Methyl Esters.- Identification of Domain Formation during sPLA2 Activity by Biophotonics.- Lipase/Esterase Activity as an Indicator of Milk Spoilage Process: A Sequential Injection Analysis Methodology.
