超低温保存・凍結乾燥プロトコル（第５版） Cryopreservation and Freeze-Drying Protocols〈Fifth Edition 2026〉

著者名：Wolkers, Willem F. (EDT)/Oldenhof, Harriëtte (EDT)

Humana（2026/09/13発売）

• 言語：ENG
• ISBN：9781071654156
• eISBN：9781071654163

Description

This detailed new edition presents the fundamental principles and emerging technologies underlying freezing and drying of biospecimens through a variety of practical protocols. After sections exploring mathematical modeling, new technologies, and the importance of standard operating procedures in biorepositories and biobanks, the book continues with cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications, including for agricultural purposes, model organisms and biodiversity conservation, reproductive and regenerative medicine, and biologics for foods and pharmaceutics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Cryopreservation and Freeze-Drying Protocols, Fifth Edition serves as an ideal resource for researchers and technical staff in academia and industry who are interested in studying the fundamentals underlying freezing and drying or in establishing practical methods for the safe storage of biological specimens.

Table of Contents

Principles Underlying Cryopreservation and Freeze-Drying of Cells.- The Principles of Freeze-Drying and Application of Analytical Technologies.- Mass Transfer in Cryopreservation at Multiple Scales: Cells, Tissues, and Organs.- Thermodynamic Principles of Emerging Cryopreservation Technologies.- Emerging Manufacturing Technologies in Freeze-Drying of Pharmaceuticals.- Advanced Rewarming Technologies for Cryopreservation.- Microfluidic Platforms for Cryopreservation.- Temperature Controlled Cryoprinting.- Standard Operating Procedures in Biorepositories and Biobanks.- Vaccine Distribution Logistics.- Cryopreservation of Bovine, Equine, and Porcine Sperm.- Standardized Cryopreservation Protocols for European Perch (Perca fluviatilis) and Pikeperch (Sander lucioperca) Semen Enabling Hatchery-Scale Fertilization.- Oocyte and Embryo Vitrification in Horses.- Cryopreservation of Bovine Blastocysts, and Blastocoel Fluid Aspiration for Large Embryos.- Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species.- Cryopreservation of Potato Shoot Tips Using PVS3 Droplet-Vitrification.- Cryopreservation by Vitrification for the Aquatic Plant Family Lemnaceae.- Isochoric Freezing of Food Products.- Cryopreservation of Rodent Ovarian Tissues and Oocytes.- Cryopreservation of Marine Invertebrates: From Sperm to Organismal Cryopreservation.- Preservation of Microorganisms for Storage in Culture Collections.- Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification Approaches.- Cryopreservation of Ovarian Cortex Tissue.- Cryopreservation of Platelets.- Cryopreserving Small Volumes of Red Blood Cells Using the High Glycerol Method.- Clinical-Grade Cryopreservation of Human Induced Pluripotent Stem Cells.- Microencapsulation for Cryopreservation of Mouse Preantral Follicles.- Protocol for Cryopreservation of Endothelial Monolayers: An Update.- Cryopreservation of Reproductive Organoids.- Protocol for Cryopreservation of Particulated Articular Cartilage by Vitrification.- Cryopreservation and Freeze-Drying of Heart Valves for Replacement Surgery.- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.- Functional and Structural Alterations Associated with Dehydration of Lactic Acid Bacteria.- Thermostabilization of Vaccines by Cryopreservation.- Thermostabilization of Vaccines by Lyophilization, Spray Drying, and Foam Drying.- Development of Freeze-Dried Formulations for Biopharmaceutical Drug Products Using a Design of Experiments Approach: A Case Study on Antibody-Drug Conjugates.- Freeze-Drying of Proteins.