Description
Antimicrobial Peptides: A Roadmap for Accelerating Discovery and Development covers the most important efforts of scientists and engineers worldwide to accelerate the process of discovery, production, and eventual market penetration of more potent antimicrobial peptides. These efforts have been fueled by emerging technologies such as artificial intelligence and data science, molecular and CFD simulations, easy-to-use process simulation packages, microfluidics, 3D-printing, among many others. Such technologies can now be implemented and scaled up quickly and at relatively low cost in low-budget production facilities, critical to moving to sustainable and marketable products worldwide.Discovering novel antimicrobial peptides rationally and cost-effectively has emerged as one of the significant challenges of modern biotechnology. Thus far, this process has been tedious and costly, resulting in molecules with activities far below those needed to address the current challenge of microbial resistance to antibiotics that takes the lives of thousands of people around the world every year. Finally, the book also highlights how multidisciplinary teams have assembled to address the challenges of manufacturing, biological testing, and clinical trials to finally reach complete translation.- Covers computational tools (including emerging artificial intelligence algorithms) and microfluidic systems for discovery and high-throughput screening of AMPs- Discusses the application of bioprocess engineering scale-up approaches for AMPs' production and purification with the aid of process simulation tools and rapid prototyping- Highlights user-centered design and formulation of products with AMPs- Describes the whole pipeline for AMPs production
Table of Contents
Introduction. Antimicrobial peptides: from the bench to the bedsideSection 1. Computational approaches1. Bioinformatic methods for the design of antimicrobial peptides2. Fundamentals of molecular dynamics for antimicrobial peptides' discovery3. Artificial intelligence for the discovery of antimicrobial peptides4. Computational tools for handling large databases of biological relevance5. Statistical analysis and data interpretationSection 2. Experimental approaches6. Chemical synthesis of peptides: conventional and novel routes7. Physicochemical and biochemical characterization8. Non-rational approaches based on microbial surface display9. Classical and emerging approximations for the screening of peptide libraries10. Scaling-up of biological production processes11. Downstream processing12. Physicochemical and biochemical characterizationSection 3. Translational studies13. Biological characterization: in vitro and in vivo studies14. Pre-clinical and clinical studies15. Formulation and product design16. Packaging, long-term stability, and usability17. Quality control and assurance
