ＲＮＡ実験法ガイド（第６版） RNA Methodologies : A Laboratory Guide for Isolation and Characterization（6）

著者名：Farrell Jr., Robert E.

Academic Press（2022/11/22発売）

• 言語：ENG
• ISBN：9780323902212
• eISBN：9780323916561

Description

RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area.This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology.- Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology- Builds from basic information on RNA techniques to in-depth protocols for specific applications- Features new chapters on RNA sequencing and RNA in situ hybridization- Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus- Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics

Table of Contents

1. RNA and the cellular biochemistry revisited 2. Creating a ribonuclease-free environment 0003. Stringency: conditions that influence nucleic acid structure4. Ribonucleic acid isolation strategies5. Isolation of polyadenylated RNA6. The truth about tissues7. Going green: RNA and the molecular biology of plants8. Quality control for RNA preparations9. cDNA: a permanent biochemical record of the cell10. RT-PCR: a science and an art form11. Quantitative polymerase chain reaction techniques12. miRNA and other noncoding RNAs13. RNA interference and gene editing14. Electrophoresis of RNA15. Photodocumentation and image analysis16. Northern analysis17. Nucleic acid probe technology18. Quantification of specific mRNAs by nuclease protection19. Analysis of nuclear RNA20. RNA in situ hybridization21. Array analysis of gene expression22. Subtractive and nonsubtractive methods for the analysis of gene expression23. Transcriptomes and bioinformatics24. RNA-seq: the premier transcriptomics tool25. RNA biomarker discovery and validation26. Functional genomics strategies27. A few RNA success stories EpilogueAppendix A: Maintaining complete and accurate records Appendix B: Converting mass to moles Appendix C: Removal of DNA from an RNA sample Appendix D: Removal of RNA from a DNA sample Appendix E: Electrophoresis: principles, parameters, and safety Appendix F: Disposal of ethidium bromide and SYBR Green solutions Appendix G: Deionization of formamide, formaldehyde, and glyoxal Appendix H: Silanizing centrifuge tubes and glassware Appendix I: Centrifugation as a mainstream tool for the molecular biologist Appendix J: Dot blot analysis Appendix K: Useful stock solutions for the molecular biologist Appendix L: Genomes and proteomes Appendix M: Common SI prefixes Appendix N: Common abbreviations Appendix O: Select suppliers of equipment, reagents, and services Appendix P: Trademark citations Glossary