Description
This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organisms. Written in the highly successful Methods inMolecular Biology series format,chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritative and cutting-edge, UnconventionalProtein Secretion: Methods and Protocols will beusefulfor all interested in the secretory pathway field as well as applications incell biology, cell development, biomedical research, and healthcare.
Table of Contents
ER to Golgi Dependent Protein Secretion: The Conventional Pathway.- UnconventionalProtein Secretion in Animal Cells.- UnconventionalProtein Secretion in Plants.- ChemicalSecretory Pathway Modulation in Plant Protoplasts.- From Cytosol to the Apoplast: The HygromycinPhosphotransferase (HYGR) Model in Arabidopsis.- Following the Time-Course of Post-Pollination Events by Transmission ElectronMicroscopy (TEM): Buildup of Exosome-Like Structures with CompatiblePollinations.- Investigating AlternativeTransport of Integral Plasma Membrane Proteins from the ER to the Golgi:Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).- Quantification of a Non-Conventional Protein Secretion:The Low Molecular Weight FGF-2 Example.- HumanPrimary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent.- A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-glycosylation in Ustilago maydis.- Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis AndQuantification.- Analysis of Yeast Extracellular Vesicles.- Exploring the LeishmaniaHydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live CellImaging Methods.- Characterization of the Unconventional Secretion of the Ebola MatrixProtein VP40.- Role andCharacterization of synuclein-γ Unconventional Protein Secretion in CancerCells.- Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF).- Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction inGrapevin.- Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation onSucrose/Deuterium Oxide (D2O) Density Cushions.
