Description
Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems.This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems.This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances.- Provides design and operation methodology for microfluidic and microfabricated materials and devices for organ-on-chip disease and safety models. This is a rapidly expanding field that will continue to grow along with advances in cell biology and microfluidics technologies.- Comprehensively covers strategies and techniques ranging from academic first principles to industrial scale-up approaches. Readers will gain insight into cell-material interactions, microfluidic flow, and design principles.- Offers three fundamental types of information: 1) design principles, 2) operation techniques, and 3) background information/perspectives. The book is carefully designed to strike a balance between these three areas, so it will be of use to a broad range of readers with different technical interests and educational levels.
Table of Contents
Part 1: Introduction1. Microfluidic Cell Culture Technologies2. Inertial microfluidics for stem cell isolation3. Microfluidic Structures for Controlling Stem Cell Microenvironments4. Microfluidic cell culture platforms with embedded nanoscale features 5. Microfluidic Vascular Networks for Engineered Tissues6. Direct Write Assembly of Organ Models7. 3D Bioprinting for Organ ModelsPart II: Organ-Specific Disease and Drug Safety Models8. Microfabricated Kidney Tissue Models9. Microfluidic Hepatotoxicity Platform10. Dynamic Gastrointestinal and Pulmonary Organ Models11. Cardiac Models12. Neural Tissue Microphysiological Systems13. Models of the Female Reproductive Tract14. Microscale Models of Inflammatory Diseases15. Blood-Retinal Barrier-on-a –chip16. Human blinking eye-on-a chip17. Circulating tumor cell cultures for evaluation of anticancer treatmentPart III: Technologies for Sensing, Multiplexed and Interconnected Organ Models18. Live Cell Analysis Under Shear Flow19. High Throughput Flow Systems for Cardiovascular Research20. Phaseguide technology for connected organ models21. Electromagnetically microactuated platforms for dynamic microphysiological systems22. Interconnected organ models for skin and liver23. Heart-Liver-Vascular Interconnected Microphysiological Systems24. Rocking Platform Technology for Interconnected Organ Models
