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Full Description
This book provides up-to-date information on the molecular diagnosis of viral diseases, including COVID-19, zika virus, human immunodeficiency virus (HIV), viral hepatitis, meningitis, and human papilloma virus (HPV). It covers global trends, the impact of viruses on society, and the need for molecular diagnostics. Initial chapters include information on popular molecular diagnostic methods and up-to-date strategies like Nucleic Acid Testing (NAT) and immunological methods, with an understanding of the challenges in their development. Further chapters emphasize emerging and next-generation diagnostic tools such as Specific High-sensitivity Enzymatic Reporter un-LOCKing (SHERLOCK), DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR), and Heating Unextracted Diagnostic Samples to Obliterate Nuclease (HUDSON), which boast a sensitivity of up to attomolar. The book also discusses high-end platforms like QIAsymphony. It is supplemented with illustrations and figures to aid conceptual understanding.
The book is relevant for researchers in virology, clinicians involved in the therapy of viral diseases, and professionals in pathology labs interested in learning about advanced molecular diagnostic approaches for viral diseases.
Contents
1. Introduction to Molecular Diagnostics and Viral Diseases.- 2. Molecular Diagnosis of COVID-19: Challenge and Pitfalls.- 3. Choice of Samples for Molecular Diagnosis of Viral Disease.- 4. Molecular and Serological Approaches for the Diagnosis of Human Orthoflaviviruses.- 5. Molecular Diagnosis of Human Immunodeficiency Virus (HIV) Infection.- 6. Recent Progress in Molecular Diagnostics of Hepatitis Viruses.- 7. Introduction to Molecular Diagnosis of Meningitis.- 8. Trends in meningitis prevalence and the need for molecular diagnosis.- 9. Molecular Diagnosis of Human Papillomavirus: Current trends and recent approaches in Molecular Diagnosis.- 10. Molecular Diagnostics for Mosquito-Borne Viral Diseases - Challenges and Emerging Concepts.- 11. Molecular Diagnosis of Enterovirus: Concepts and Challenges.- 12. Advanced Molecular Techniques in Diagnosis of Viral Diseases.- 13. Molecular Diagnosis beyond Nucleic Acid Testing (NAT).
