Full Description
This is the first experimental protocol book that covers the differentiation of bone marrow-derived stem cells (BMSCs) into specific cell types, targeted at the undergraduate and graduate student level. The 19 chapters deal with the differentiation methods using small molecules, cytokines and polymeric scaffolds.BMSCs are pluripotential in that they not only act as myelo-regenerative and supportive cells, but can also differentiate into almost any kind of cells in our body. In addition, when implanted in vivo, they could help repair multiple tissues such as blood vessels, heart, liver and so on.For the differentiation of BMSCs, many methods have been introduced to adjust their microenvironment (chemical and physical cues), including chemical induction methods using large or small molecules and pellet culture; mechanical stimulation induction methods using cyclic mechano-transduction or ultrasonication; cytokine-released method using scaffolds; and so on.
Contents
Introduction; Chondrogenic Differentiation of Rat BMSCs in Hydrogel; Protocol of Chondrogenesis from BMSCs using TGF-b Loaded Alginate Bead; Protocol of Chondrogenesis from Human BMSCs By Pellet Culture; Protocol of Chondrogenesis from BMSC on a Porcine Chondrocytes-derived Extracellular Matrix Scaffold; Protocol of Chondrogenesis of MSC by Ultrasonication; Protocol of Chondrogenesis of BMSC to Chondrocyte Using Chitosan-Modified Poly(L-Lactide-co-ε-Caprolactone) Scaffolds; Protocol of Osteogenesis of BMSCs using Hydroxyapatite/Tricalciumphosphate Scaffold; Protocol of Osteogenesis from BMSC cultured with Dexamethasone-Loaded Dendrimer Nanoparticles onto Ceramic and Polymeric Scaffolds: In Vivo Studies; Protocol for Osteogenesis of BMSC in Calcium Phosphate Ceramics; Protocol of Osteoblastic Differentiation of BMSC in Biodegradable Scaffolds composed of Gelatin and ε-Tricalcium Phosphate; Protocol of Cardiomyogenic Induction of hMSCs on Dendrimer-immobilized Surfaces displaying with d-Glucose; Protocol of Cardiomyocyte Differentiation of BMSC by Small Molecules; Protocol for the Differentiation of BMSCs to a Smooth Muscle Cell for the Application of Engineering Small Diameter Blood Vessels; Protocol of Schwann Cell Differentiation of BMSC by Direct Co-culture Method using Insert System; Protocol of Neurogenesis of BMSC Using β-Mercaptoethanol Released System from β-Mercaptoethanol-Loaded PLGA Film; Protocol of Neural Differentiation from BMSCs using bFGF and Laminin-coating Plate; Protocol of Differentiation of Retinal Pigment Epithelial-like Cells from BMSC using Co-culture Method; Protocol of Differentiation of Olfactory Ensheathing Cells from BMSCs by Insert and Conditioned Media System; Protocol of the Differentiation of BMSC to Corneal Endothelial Cells by Direct and Indirect Co-culture.
