Full Description
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
Contents
A Brief Introduction to Recombinant DNA Technology.- Cloning and Gene Manipulation.- Selection of Cloning and Expression Plasmid Vectors.- Transformation and Protein Expression.- Introduction to Recombinant Protein Purification.- Protein Purification by Affinity Chromatography.- Protein Purification by Ion Exchange Chromatography.- Gel Filtration Chromatography.- Protein Purification by Reversed Phase Chromatography and Hydrophobic Interaction Chromatography.- Purification of Difficult Proteins.- Protein Quantitation and Detection.
