Full Description
The book covers the essential practical techniques of flow cytometry in detail. It is divided into two sections: The first section includes the basic practical techniques of flow cytometry in cytology samples. Chapters under this section provide detailed description of the sampling technique, processing, acquisition of the sample, instrumentation and basic principles of flow cytometry. The second section elucidates clinical applications of flow cytometry. Chapters cover the flow cytometry applications in various haematolymphoid neoplasms, tumors of solid organs and body fluid samples. The flow cytometry findings of different tumors are described with the help of multiple colored cytology microphotographs, flow cytometry graphs, boxes, and tables. In addition, it also describes other ancillary techniques in those neoplastic lesions.
The book helps practicing pathologists, technical staff and post graduate students to understand flow cytometry findings of the haematolymphoidneoplasms and solid tumor with special emphasis on cytology along with advanced technique. This book will help the students to interpret flow cytometry graphs.
Contents
Part I: Technical aspects.- Introduction and history of flow cytometry.- Basic principles of flow cytometry and instrumentation.- Specimen preparation and data acquisition.- Interpretation of graphs and quality control.- Fluorescent probes and different useful markers for flow cytometry.- Nuclei acid dye for flow cytometry.- Part II: Applications of flow cytometry.- Application of flow cytometry in lymphoid neoplasms.- Application of flow cytometry in body cavity fluids.- Application of flow cytometry in solid tumors.- Cell cycle analysis, DNA flow cytometry and ploidy analysis.- Future applications of flow cytometry.- Practical cases in flow cytometry.
