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Full Description
The growing scale of plant-based chemicals for industrial use has generated considerable interest in developing methods to meet their desired production levels. Among various available strategies for their production, the development of Agrobacterium rhizogenes mediated hairy root cultures (HRCs) is generally considered the most feasible approach. Additionally, several proof-of-principle experiments have demonstrated the practical feasibility of HRCs in the plant-based remediation of environment pollutants, biotransformation of important compounds, and production of therapeutic proteins.
Given that hairy root biotechnology has now been recognized as a promising and highly dynamic research area, this book offers a timely update on recent advances, and approaches hairy roots as a multifaceted biological tool for various applications. Further, it seeks to investigate the loopholes in existing methodologies, identify remaining challenges and find potential solutions by presenting well thought-out scientific discussions from various eminent research groups working on hairy root biotechnology.
This book provides detailed conceptual and practical information on HRC-based research, along with relevant case studies. The content is divided into three broad sections, namely (i) Hairy Roots and Secondary Metabolism, (ii) Progressive Applications, and (iii) Novel Approaches and Future Prospects. By informing the research and teaching community about the major strides made in HRC-based interventions in plant biology and their applications, the book is sure to spark further research in this fascinating field.
Contents
Part I: Hairy Roots and Secondary Metabolism.- Progress and Prospects of Hairy Root Research.- A Critical Review on Biotechnological Interventions for Production and Yield Enhancement of Secondary Metabolites in Hairy Root Cultures.- pRi-Transformed Plants as a Source of Secondary Metabolites.- Biotechnological Intervention of Hairy Roots of Tropane Alkaloid Bearing Plants.- Hairy Root Cultures for Monoterpene Indole Alkaloid Pathway: Investigation and Biotechnological Production.- Stress Induced Metabolite Production Utilizing Plant Hairy Roots.- Bioreactor Design and Analysis for Large Scale Plant Cell and Hairy Root Cultivation.- Part II: Progressive Applications. - Hairy Root Mediated Biotransformation: Recent Advances and Exiting Prospect.- Hairy Roots as Bioreactors for the Production of Biopharmaceuticals.- Phytoremediation of Persistent Organic Pollutants (POPs) Utilizing Transgenic Hairy Root Cultures: Past and Future Perspectives.- Use of Hairy Root System tostudy Signalling Pathways During Nodule Formation.- Hairy Roots as a Tool for the Functional Analysis of Plant Genes.- Part III: Novel Approaches and Future Prospects.- An Update on Transcriptome Sequencing of Hairy Root Cultures of Medicinally Important Plants.- Strategies for Monitoring and Modeling Growth of Hairy Root Cultures: An in Silico Perspective.- Engineering in Hairy Roots using CRISPR/Cas9 Mediated Editing.
