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Full Description
Molecular biology, particularly molecular genetics, is among the newest and most powerful approach in modern photosynthesis research. Development of molecular biology techniques has provided new methods to solve old problems in many biological disciplines. Molecular biology has its greatest potential for contribution when applied in combination with other disciplines, to focus not just on genes and molecules, but on the complex interaction between them and the biochemical pathways in the whole organism. Photosynthesis is surely the best studied research area in plant biology, making this field the foremost candidate for successfully employing molecular genetic techniques. Already, the success of molecular biology in photosynthesis has been nothing short of spectacular. Work performed over the last few years, much of which is sum marized in this volume, stands in evidence. Techniques such as site-specific mutagenesis have helped us in examining the roles of individual protein domains in the function of multiunit complexes such as the enzyme ribulose-l ,5-bisphos phate carboxylase/oxygenase (RUBISCO) and the oxygen evolving photo system (the photosystem II). The techniques of molecular biology have been very important in advancing the state of knowledge of the reaction center from the photosynthetic bacteria whose structure has been elegantly deduced by H. Michel and 1. Deisenhofer from the X-ray studies of its crystals.
Contents
I Genes and genetics.- 1 Nicotiana chloroplast genes for components of the photosynthetic apparatus..- 2 Gene organization and newly identified groups of genes of the chloroplast genome from a liverwort Marchantia polymorpha..- 3 Introns in chloroplast protein-coding genes of land plants..- 4 Pea chloroplast tRNALys (UUU) gene: transcription and analysis of an intron-containing gene..- 5 Transposon mutagenesis of nuclear photosynthetic genes in Zea mays..- 6 Using bacteria to analyze sequences involved in chloroplast gene expression..- 7 Binding, uptake and expression of foreign DNA by cyanobacteria and isolated etioplasts..- II Light harvesting systems.- 8 The puf Operon regoin of Rhodobacter sphaeroides..- 9 Characterization of phycobiliprotein and linker polypeptide genes in Fremyella diplosiphon and their regulated expression during complementary chromatic adaptation..- 10 Photoregulation of gene expression in the filamentous cyanobacterium Calothrix sp. PCC 7601: light-harvesting complexes and cell differentiation..- 11 Regulation of cyanobacterial pigment-protein composition and organization by environmental factors..- 12 The major light-harvesting complex of Photosystem II: aspects of its molecular and cell biology..- 13 Regulation and expression of the multigene family coding light-harvesting chlorophyll a/b binding proteins of Photosystem II..- III Reaction centers; herbicide resistance.- 14 Reaction centers from three herbicide-resistant mutants of Rhodobacter sphaeroides 2.4.1: sequence analysis and preliminary characterization..- 15 Molecular genetics of herbicide resistance in cyanobacteria..- 16 Genetic analysis of two new mutations resulting in herbicide resistance in the cyanobacterium Synechococcus sp. PCC 7002..- 17 Nucleotide sequence of the genes encoding cytochrome b-559 from the cyanelle genome of Cyanophora paradoxa..- 18 Protein composition of the Photosystem II core complex in genetically engineered mutants of the cyanobacterium Synechocystis sp. PCC 6803..- 19 The QBsite modulates the conformation of the Photosystem II reaction center polypeptides..- 20 Photoregulation of psbA transcript levels in mustard cotyledons..- 21 The molecular mechanism of the Bicarbonate effect at the Plastoquinone reductase site of Photosynthesis..- 22 Photosystem I complex..- IV Electron transport components; ATP synthase.- 23 Synthesis and assembly of the cytochrome b-f complex in higher plants..- 24 Genes encoding ferredoxins from Anabaena sp. PCC 7937 and Synechococcus sp. PCC 7942: structure and regulation..- 25 Structure, organization and expression of cyanobacterial ATP synthase genes..- 26 The chloroplast genes encoding subunits of the H+-ATP synthase..- V Carbon; Rubisco.- 27 Structural gene regions of Rhodobacter sphaeroides involved in CO2 fixation..- 28 Uptake and utilization of inorganic carbon by cyanobacteria..- 29 Synthesis and assembly of bacterial and higher plant Rubisco subunits in Escherichia coli..- 30 Organization and expression of the genes encoding ribulose-1,5-bisphosphate carboxylase in higher plants..- 31 Cloning, expression and directed mutagenesis of the genes for ribulose bisphosphate carboxylase/oxygenase..- 32 The Rubisco subunit binding protein..- 33 The value of mutants unable to carry out photorespiration..- 34 Gene expression during CAM induction under salt stress in Mesembryanthemum: cDNA library and increased levels of mRNA for phosphoenolpyruvate carboxylase and pyruvate orthophosphate dikinase..- VI Protein transport; synthesis.- 35 Transport of proteins into chloroplasts..- 36 Protein transport towards the thylakoid lumen: post-translational translocation in tandem..- 37 Recent developments in chloroplast transport..- 38 Protein synthesis by isolated chloroplasts..
