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Full Description
This book provides a general introduction of plant tissue culture followed by specific applications of biotechnology in regeneration of rice (Oryza sativa), Maize, Eucalyptus, hot pepper, guava (Psidium guajava L.) stone fruit (Pinus pinea) and compares the features of in vitro grown plants to in vivo plants, Transgenic plants production and application, generating marker-free transgenic plants, genetic engineering and metabolic engineering of plants, molecular farming, abiotic stress tolerance, transgenic in floriculture and ornamental plants, celery, Secondary metabolite production with special reference to sennoside, genetic transformation of potato and biosafety concerns, bioinformatics and its application to crop improvement, Intellectual property rights, biotechnological aspects of secondary metabolite production, application of biotechnology in pharmaceutical sciences and production of recombinant proteins, cyclotides, Hypericum perforatum and Gentiana punctata provide a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. Besides covering basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, gene technology and secondary metabolite production. The book will prove useful to both students and researchers of biotechnology, agriculture, horticulture, forestry as well as for the industry.
Contents
1. Tissue Culture: Historical Perspectives and Applications; Section I: In Vitro Plant Regeneration; 2. High Frequency Plant Regeneration via Multiple Shoot Induction from Shoot Apex in Indica Rice (Oryza sativa L.) Varieties; 3. Somatic and Gametic Embryogenesis in Maize: Cell Biology and Applications; 4. Somatic Embryogenesis in Eucalyptus - An Update to 2009; 5. Increased Embryo Production by Manipulation of Pretreatment Materials and Media in Isolated Microspore Culture of Hot Pepper (Capsicum annuum L.). 6. Biotechnological Advancement in Getting In Vitro Regeneration and Mass Multiplication in Guava (Psidium guajava L.): A Success Story in Fruit Trees; 7. In Vitro Propagation of Stone Pine (Pinus pinea L.) Molecular and Physiological Bases; 8. Morphological, Anatomical and Physiological Characteristics of in vitro Grown Plants in Comparison to ex vitro Grown Plants; Section II: Transgenic Plants and their Applications; 9. Production of Transgenic Plants and Their Application in Biotechnology; 10. Practical Application of Genetic Transformation; 11. Generating Marker-free Transgenic Plants; 12. Role of recA/Rad51 Gene Family in Homologous Recombination, Repair and Genetic Engineering of Transgenic Plants; 13. Metabolic Engineering in Plants; 14. Molecular Farming for Production of Biologically Important Molecules in Plants; 15. Improving Crop Tolerance to Abiotic Stresses by Plant Biotechnology; 16. Transgenic Floricultural and Ornamental Plants; 17. Flux in Morphology and GUS Expression in Somatic Embryogenic-Derived Populations of Transgenic Celery; 18. The Potato (Solanum tuberosum L.): An Overview of Genetic Transformation and Related Biosafety Issues; 19. Clean DNA Transformation of Rice Using Minimal Gene-Expression-Cassettes to Minimize Biosafety Concerns; 20. Bioinformatics Application to Crop Improvement; 21. Intellectual Property Rights, Biopiracy & Patent Revocation; Section III: Secondary Metabolites and medicinal plants; 22. Biotechnological Aspects of Secondary Metabolite Production; 23. Applications of Plant Biotechnology in Pharmaceutical Sciences: A Critical Review; 24. Production of Recombinant Proteins in Plants for Pharmaceutical Use; 25. Intraspecific Variation of Plant Secondary Metabolites; 26. Optimisation Steps Towards Bioprocess Development for the Production of Cyclotides; 27. Genetic (In)stability in Hypericum perforatum L.: Physiological and Induced Sources of Genetic Variation; 28. Biotechnology of Endangered Medicinal Plants: Spotted Gentian, Gentiana punctata L.; 29. Direct Shoot Regeneration of Cassia senna for Sustainable Production of Anthraquinone Glycoside-Sennoside; Index; Author Index.
