基本説明
Special Issue: 'Cells Tissues Organs 2004, Vol. 177, No. 3'.
Full Description
Significant progress has been made in gene technology and skin gene therapy in the last few years. Besides the development of suitable animal models, novel molecular technologies such as small inhibitory RNA are discussed, which enable the modification of sequence-specific gene expression. Progress is reported in the field of DNA vaccination against HIV-1 as well as in the correction of xeroderma pigmentosum using retroviral-based gene transfer. In addition to gene technology and skin gene therapy, recent advances in molecular diagnostics of malignancy are described. Microarray-based gene expression profiling is a powerful technology with which up to 22,000 genes can simultaneously be analyzed in a given tissue. It is used today by several groups to characterize malignant melanoma and other tumors on a molecular level, identifying important dysregulated pathways and gene products and comparing them with those of normal human melanocytes and benign nevi. Providing an overview of the latest techniques and achievements in the field, this publication is recommended to dermatologists, geneticists, bioengineers, cell biologists and immunologists, and anyone interested in learning more about new molecular approaches in biomedicine.
Contents
Advances in Molecular Diagnostics; Rapid Identification of Dysregulated Genes in Cutaneous Malignant Melanoma Metastases Using cDNA Technology: Mirmohammadsadegh, A.; Baer, A.; Nambiar, S.; Bardenheuer, W.; Hengge, U.R. A Genomic- and Proteomic-Based Hypothesis on the Eclectic Effects of Systemic Interleukin-2 Administration in the Context of Melanoma-Specific Immunization: Paneill, M.C.; Martin, B.; Nagorsen, D.; Wang, E.; Smith, K.; Monsurro, V.; Marincols, F.M. Progress in Molecular Technologies; Exogenous siRNA Mediates Sequence-Independent Gene Suppression by Signaling through Toll-Like Receptor 3: Karlko, K.; Bhuyan, P.; Capodici, J.; Ni, H.; Lubinski, J.; Friedman, H.; Weissman, D. Progress in the Use of Adeno-Associated Viral Vectors for Gene Therapy: Buning, H.; Braun-Falco, M.; Hallek, M. Topical Colchicine Selection of Keratinocytes Transduced with the Multidrug Resistance Gene (MDR1) Can Sustain and Enhance Transgene Expression in vivo: Pfutzner, W.; Vogel, J.C. Inducible Mouse Models for Inherited Skin Diseases: Implications for Skin Gene Therapy: Arin, M.J.: Roop, D.R. Progress towards DNA Vaccination and Skin Gene Therapy; Genetic Immunization with Multiple HIV-1 Genes Provides Protection against HIV-1/MuLV Pseudovirus Challenge in vivo: Hinkula, J.; Rollman, E.; Lundholm, P.; Benthin, R.; Okuda, K.; Wahren, B. Experimental Leishmania major Infection Suppresses HIV-1 DNA Vaccine Induced Cellular Immune Response: Robinson, T.M.; Nelson, R.; Artis, D.; Scott, P.; Boyer, J.D. Xeroderma pigmentosum: From Symptoms and Genetics to Gene-Based Skin Therapy: Magnaldo, T.; Sarasin, A. Author Index; Subject Index
