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Full Description
The application of fluorescence in drug discovery, high-throughout screenings in genomics and proteomics is and will be evidently successful. The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. Probably the most significant breakthrough in fluorescence is its use in detection of single molecules and even of their real-time dynamics. Also, probing inside living cells has become a hot topic in the life sciences. This topic book reflects the updates of scientific progress presented by frontline researchers.
Contents
1 Advanced Luminescent Labels, Probes and Beads and their Application to Luminescence Bioassay and Imaging.- 2 Fluorescence Spectral Engineering - Biophysical and Biomedical Applications.- 3 Fluorescence Nanometrology in Sol-Gels.- 4 Integrated Supramolecular Systems: From Sensors to Switches.- 5 Ratiometric Probes: Design and Applications.- 6 Binding of Ethidium to Yeast tRNAPhe: A New Perspective on an Old Bromide.- 7 Experimental Aspects of DNA Computing by Blocking: Use of Fluorescence Techniques for Detection.- 8 Multiparametric Detection of Fluorescence Emitted from Individual Multichromophoric Systems.- 9 Fluorescence Intensity Distribution Analysis (FIDA) and related fluorescence fluctuation techniques: theory and practice.- 10 Single Molecule Reactions of the Enzyme LDH and of Restriction Endonucleases in the Fluorescence Microscope.- 11 Monitoring ?-Subunit Movement in Reconstituted Single EFoF1 ATP Synthase by Fluorescence Resonance Energy Transfer.- 12 Application of the Wavelength-selective Fluorescence Approach to Monitor Membrane Organization and Dynamics.- 13 Fluorescence Approaches for the Characterization of the Peripheral Membrane Binding of Proteins Applied for the Blood Coagulation Protein Prothrombin.- 14 Assessment of Membrane Fluidity in Individual Yeast Cells by Laurdan Generalised Polarisation and Multi-photon Scanning Fluorescence Microscopy.- 15 Formation of Higher Order Signal Transduction Complexes as Seen by Fluorescence Spectroscopy.- 16 Mechanisms of the Modulation of Membrane Interfacial Enzyme Catalysis by Non-lamellar Forming Lipids: Comparison with the Behavior of a Fluorescent Probe in Membranes.- 17 Emission Spectroscopy of Complex Formation between Escherichia coli Purine Nucleoside Phosphorylase (PNP) and Identified TautomericSpecies of Formycin Inhibitors Resolves Ambiguities Found in Crystallographic Studies.- 18 Fluorescence Lifetime Imaging Implemented with Resonant Galvanometer Scanners.- 19 Spectral Imaging of Single CdSe/ZnS Quantum Dots Employing Spectrally- and Time-resolved Confocal Microscopy.- 20 Imaging of Oxidative Stress in Plant Cells by Quantitative Fluorescence Microscopy and Spectroscopy.- 21 The Biomedical Use of Rescaling Procedures in Optical Biopsy and Optical Molecular Imaging.- 22 Looking into a Living Cell.- 23 Expression of Multicolor Fluorescent Fusion Proteins in Zebrafish Cell Cultures: A Versatile Tool in Cell Biology.
