Description
(Text)
The aim of this work was to study matrix metalloproteinase-26 (MMP-26) and tissue inhibitor of metalloproteinases-4 (TIMP-4) in the human endometrium. Both these enzymes may be implicated in the implantation process. Trophoblast tissue from human, as well as mice embryo produces pro-MMP-9. Active MMP-9 is a proteolytic enzyme with a broad substrate specificity. MMP-26 is an affective activator of pro-MMP-9, and TIMP-4 is a strong inhibitor of MMP-26.We have shown that the endometrial expression of MMP-26 and TIMP-4 genes is maximal in mid-cycle. The expression pattern of both genes suggests their up-regulation by estrogen, and down-regulation by progesterone. This is supported by our findings of estrogen response elements upstream the coding sequences of both genes. Our findings suggest that TIMP-4 is produced in the stroma only, and finally secreted to the uterine fluid. Results of MMP-26 and TIMP-4 expression in hyperplastic tissue are in agreement with estrogen mediated regulation of these genes. MMP-26 and TIMP-4 expression in endometrial cancer decreases with the loss of histological differentiation.
(Author portrait)
Medical researcher;born in Uherske Hradiste,CzechRepublic (CR); M.D.UJEP Brno,CZ,1989;Ph.D.PalackyUniversity,Olomouc,CR,2001;Head Physician,Obst/Gyn,UniversityHospital, Olom.,CR,since 2004;Assoc.Prof.Pal.Univ.,Olom.,CR,2005;Ph.D.LundUniv.,Sweden,2006;Deputy Dean study affairs,Pal.Univ.,Olom.,CR,since 2007; Married,Two children.
(Author portrait)
Pilka Radovan Medical researcher;born in Uherske Hradiste,Czech Republic (CR); M.D.UJEP Brno,CZ,1989;Ph.D.Palacky University,Olomouc,CR,2001;Head Physician,Obst/Gyn,University Hospital, Olom.,CR,since 2004; Assoc.Prof.Pal.Univ.,Olom.,CR,2005;Ph.D.Lund Univ.,Sweden,2006;Deputy Dean study affairs, Pal.Univ.,Olom.,CR,since 2007; Married,Two children.
