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used. Generally in the development of a process, research activity will start with the physiology of the immobilized cells and be concluded by scale-up and application. Guidelines for the different phases in research are given below. Springer Lab Manual about immobilized cells The Springer Lab Manual about immobilized cells is structured in such a way that guidelines that resulted from the conference 'Immobilized Cells: Basics and Applications' were followed and translated into some practical methods. As for the guidelines for immobilized cells a structure was chosen in which general aspects of immobilization were covered, the kinetics were described and engineering aspects were covered. Besides laboratory meth- ods some simple calculation procedures have been given in this lab man- ual. The combination of the practical methods with the calculations pro- vide a tool for carrying out research with immobilized cells. The lab manual concludes with some recent, promising and practical cases of immobilized cells as examples of applications with potential.
Contents
Characterization of immobilized cells.-Description of the support material.-Description of the immobilisation procedure.-Measurement of density, particle size and shape of support.-Mechanical stability of the support.-Diffusion coefficients of metabolites.-Quantitity of biomass immobilized, determination of biomass concentration.-Kinetics of the suspended cells.-Diffusion limitation.-Micro-electrodes.-Biomass gradients.-NMR and immobilized cells.-Immobilisation at large scale by dispersion.-Immobilization at large scale by the resonance nozzle technique.-External mass transfer.-Liquid fluidization of gel bead particles.-Gradients in liquid, gas or solid fractions.-Support material stability at the process conditions.-Immobilized cells in food technology: Storage stability, sensitivity to contamination and safety.- And other contributions.-Subject index
