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Full Description
As with chemical reactions in general, turnover rates of enzyme-catalyzed reactions increase with temperature. For industrial applications, therefore, understanding and influencing the stability of enzymes above their usual range of biological temperatures is crucial for economic reasons. The editor has compiled the first overview of thermostable enzymes in this reference book. Leading experts outline strategies for the search for thermostable enzymes and for engineering thermostability.
Contents
Contents: Protein Structure and Stability.- Isolation and Purification of Proteins.- Thermophilic Microorganisms: The Biological Background for Thermophily and Thermoresistance of Enzymes.- Need for Thermostability, its Benefits and Main Strategies for Thermostabilization.- Models for Enzyme Deactivation.- Measuring the Conformational Stability of Enzymes.- Mechanisms of Irreversible Thermoinactivation and Medium Engineering.- Stabilization of Enzymes with Soluble Additives - Chemical Modification and Chemical Crosslinking for Enhancing Thermostability of Enzymes.- Immobilization Techniques for Altering Thermal Stability of Enzymes.- Protein Engineering for Thermostabilization.
