Contents
1 Introduction.- 2 Materials and Methods.- 2.1 Animals and Animal Experiments.- 2.2 Fixation.- 2.3 Postfixation, Osmium-Low Ferrocyanide Reaction.- 2.4 Dehydration and Embedding.- 2.5 Cytochemistry of Carbohydrates in Ultrathin Sections.- 2.5.1 PTA-HCL Reaction.- 2.5.2 PA-TCH-Ag Reaction.- 2.5.3 mPA-TCH-Ag Reaction.- 2.5.4 HID-TCH-Ag Reaction.- 2.6 Electron Microscopy.- 3 Results.- 3.1 Lysosomes.- 3.1.1 Heterolysosomes of Untreated Animals.- 3.1.2 Heterolysosomes Induced by Triton WR-1339.- 3.1.3 Autophagic Vacuoles.- 3.1.4 Multivesicular Bodies.- 3.1.5 Eosinophilic Leukocytes.- 3.2 Zymogen Granules of Exocrine Glands.- 3.2.1 Submandibular Gland.- 3.2.2 Parotid Gland.- 3.2.3 Pancreas.- 3.2.4 Paneth Cells.- 3.3 Secretory Granules of Endocrine Glands.- 3.3.1 Adenohypophysis.- 3.3.2 Neurohypophysis.- 3.3.3 Gastroenteropancreatic Endocrine Cells.- 3.3.4 Adrenal Medulla.- 3.4 Mast Cell Granules.- 3.5 Mitochondria and Peroxisomes.- 3.6 Specificity Controls.- 4 Discussion.- 4.1 Critique of the Methods.- 4.1.1 Pretreatment of the Tissue.- 4.1.2 Cytochemieal Methods for the Demonstration of Carbohydrates.- 4.2 Evaluation of the Cytochemieal Results.- 4.2.1 Inner Membrane Coat of Lysosomes and Secretory Granules.- 4.2.2 Matrix of Lysosomes and Secretory Granules.- 4.2.3 Mitochondria, Peroxisomes, and Golgi Apparatus.- 4.3 Concluding Remarks.- 5 Summary.- References.
