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Description
(Short description)
A comprehensive and up-to-date overview of microarray detection methods for highly dangerous BSL3 and BSL4 agents, this is a must-have for all laboratories working with these pathogens as well as public health agencies focused on bioterrorism.
(Text)
Written by leading experts in the field as part of an interdisciplinary pan-European research program funded by the EU, the results provided in this booklet provide a unique and comprehensive overview of how microarray technology can be used in safely tracking the most highly dangerous pathogens. A must-have for public health agencies focused on bioterrorism as well as all laboratories working with BSL3 and/or BSL 4 agents.
(Table of content)
Preface (Kostic, Butaye, Schrenzel)
Introduction on Microarray-based Detection Methods (Schrenzel, Kostic, Bodrossy, Francois)
METHODS
Long-oligonucleotide Microarray-based Microbial Detection (Kostic, Bodrossy)
Sequence-specific End-labelling of Oligonucleotides (SSELO) (Kostic, Bodrossy)
Non-cognate Approaches for Pathogen Detection on Microarrays (Schrenzel, Francois, Hernandez, Hibbs, Huyghe, Charbonnier)
Patterning Techniques for Array Platforms (Piskin, Garipcan, Demirel, Caglayan)
Probe Immobilization Techniques in Array Technologies (Piskin, Caglayan, Duman)
IDENTIFICATION
Low-Cost-and-Density (LCD)-Microarrays - a Novel Technique for Identification and Typing of Microorganisms (Frangoulidis, Heiser, Landt, Meyer)
DNA Microarray Technique for Detection and Identification of Viruses Causing Encephalitis and Hemorrhagic Fever (Nordstroem, Falk, Nilsson, Lundkvist)
Microarrays for genomotyping of pathogens (Kieboom, Voskamp, Broekhuijsen)
TYPING
Single Nucleotide Polymorphisms (SNPs) as Targets for DNA-array Based Identification and Typing of BSL3 Bacteria (Wattiau, Vos, Fretin)
Use of a Microchip to Detect Antibiotic Resistance Genes in Bacillus anthracis (Perreten, Frey)
QUALITY CONTROL
Progress Toward Development of Microarrays for Routine Diagnostic Use for Detection of Select High Risk Pathogens (Kempsell, Shah, Sherwin, Vipond, Silman)



