Full Description
This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.
Contents
PERSPECTIVES IN PROTEOMICS SAMPLE PREPARATION
Introduction
General Aspects of Sample Preparation for Comprehensive Proteome Analysis
Proteomics: A Philosophical Perspective
MASS SPECTROMETRY
A Practical Guideline to Electrospray Ionization Mass Spectrometry for Proteomics Application
Sample Preparation for the Application of MALDI Mass Spectrometry
Sample Preparation for Label-Free Proteomic Analyses of Body Fluids by Fourier Transform Ion Cyclotron Mass Spectrometry
Sample Preparation for Differential Proteome Analysis: Labelling Technologies for Mass Spectrometr
Determining Protein Localization within Subcellular Compartments using Stable IsotopeTagging
ELECTROPHORESIS
Sample Preparation for Two-Dimensional Gel Electrophoresis
Sample Preparation for Native Electrophoresis
Sample Preparation for LC-MS/MS using Free Flow Electrophoresis
Sample Preparation for Proteomics
OPTICAL METHODS
High-Throughout Proteomics: Spinning Disc Interferometry (SDI)
Optical Proteomics on Cell Arrays
Sample Preparation by Laser Microdissection and Catapulting for Proteome Analysis
Sample Preparation for Flow Cytometry
CHROMATOGRAPHY
Sample Preparation for HPLC-Based Proteome Analysis
Sample Preparation for Two-Dimensional Phosphopeptide Mapping and Phosphoamino Acid Analysis
STRUCTURAL PROTEOMICS
Exploring Protein-Ligand Interactions by Solution NMR
Sample Preparation for Crystallography
INTERACTION ANALYSIS
Sample Preparation for Protein Complex Analysis by the Tandem Affinity Purification (TAP)
Methods
Exploring Membrane Proteomes
POST-TRANSLATIONAL MODIFICATIONS
Sample Preparation for Phosphoproteome Analysis
Sample Preparation for Analysis of Post-Translational Modifications: Glycosylation
SPECIES-DEPENDENT PROTEOMICS
Sample Preparation and Data Processing in Plant Proteomics
Sample Preparation for MudPIT with Bacterial Protein Samples
Sample Preparation for the Cell-Wall Proteome Analysis of Yeast and Fungi
THE HUMAN PROTEOSOME
Clinical Proteomics: Sample Preparation and Standardization
Stem Cell Proteomics
BIOINFORMATICS
Bioinformatics Support for Mass Spectrometric Quality Control
Use of Physio-Chemical Properties in Peptide and Protein Identification
