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Full Description
This updatable reference work gives a comprehensive overview about how to fully exploit the potential of bioimaging to characterize tissue regeneration holistically and to gain complementary, multi-scale information about relevant structure, function, dynamics and molecular composition. To keep pace with the highly dynamic nature of the field and fast-developing imaging technology, it is essential to allow the book to be updated and dynamic. It guides the reader through real-case biomedical applications organized by chapter and gives a tissue-specific overview on how to best assess parameters of interest. Additionally, all imaging techniques, including emerging modalities, are explained in greater detail in the Appendix, which serves as a reference chapter and enables the reader to understand the physical principles behind each modality. The book provides a unique overview of available biological and (pre)clinical imaging techniques in the field and enables the reader to understandtheir benefits and limitations for real-case applications. The reader will be equipped with a comprehensive tool to properly chose the most promising technique for her/his biomedical research question - and respond to novel trends due to the dynamic nature of the book. The book is of high interest to all researchers, physicians, physicists and life scientists working in the field to deepen their theoretical and practical knowledge about bioimaging and about the wide range of tissue-specific biomedical applications. The continuously updated e-book will be a valuable reference in the field for bioimaging.
Contents
Fluorescence Microscopy of the Cell and Cell Interactions to Study Dynamic Cellular Processes.- Live Cell Imaging and in vivo Cell Tracking in Tissues.- Nonlinear Microscopy to study arthroscopy.- Synchronization to Visualization: Dissecting Myogenesis and Regeneration Using Correlative Light and Electron Microscopy (CLEM).- Raman Imaging for Biomedical Applications.- X-ray-based 3D histo(patho)logy of biological tissues using contrast-enhanced microCT.- Simple and Intelligible Microscopy of Living Cells with High Resolution and Sufficient Statistics.- Correlated Multimodal Imaging in Bone of Regeneration - A Showcase of Bisphosphonate-Treated Murine Jawbones.- Chemical Imaging in Bone and Cartilage Regeneration.- Quantitative Microstructural Imaging and Mechanics of Cartilage Using Experimental and Computational Approaches.- Osteoarthritis Imaging Using MRI.- Correlative Multimodal Imaging for Multiscale Analysis of Complex Biological Systems: Validation Methods and Performance Analysis.- HREM for Mesoscopic 3D Histology.- X-Ray Physics and Micromechanics-Guided Intravoxel Analysis of microCT-Imaged Hard Tissue Engineering Scaffolds and Bone.- Combined Imaging and Soft and Complex 3D Bioprinting using Light Sheet Fluorescence Microscopy.