Full Description
This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.
Contents
1. Overview to animal cell culture.- 2. Cell culture laboratory.- 3. Good lab practices and bio-safety measures.- 4. Managing sterility in the lab.- 5. Media and buffer preparation for cell culture.- 6. Properties of cultured cells and selection of culture media.- 7. Selection and maintenance of cultured cells.- 8. Inoculation and passaging of adherent cells.- 9. Counting of cells.- 10. Cryopreservation of cell lines.- 11. Resuscitation of frozen cell line.- 12. Isolation and culturing of cells from different tissues.- 13. Differentiation, transformation and immortalization of cells.- 14. Co-culture techniques.- 15. 3d cell culture techniques.- 16. Stem cell culture techniques.- 17. Identification and removal of biological contamination in the media and cell suspensions.- 18. Analysis of cell growth kinetics in suspension and adherent types cell line.- 19. Applications of animal cell culture based assays.- 20. Ethical issues in animal cell culture.- 21. Common troubleshooting methods in cell culture techniques.
