Full Description
Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine.
Contents
List of figuresList of tablesAcknowledgmentsAbout the authorsChapter 1: Characteristics of stem cellsAbstract:1.1 Introduction1.2 Stem cell specialization1.3 Stem cell types and functions1.4 Signaling dynamics and stem cell pluripotency1.5 Novel technologies and clinical applications1.6 ReferencesChapter 2: Stem cell culture: mimicking the stem cell niche in vitroAbstract:2.1 Introduction2.2 Stem cell niches2.3 Niche components and structures2.4 Engineering the microenvironment2.5 Conclusions and future directions2.6 ReferencesChapter 3: Bioreactors for stem cell cultureAbstract:3.1 Introduction3.2 Stem cell isolation and characterization3.3 Critical issues in stem cell bioprocess development3.4 Bioreactor design and operation3.5 Bioreactor configurations3.6 Bioprocess monitoring and control3.7 Stem cell cultivation in bioreactors3.8 Conclusions and final remarks3.9 ReferencesChapter 4: Stem cell separationAbstract:4.1 Introduction4.2 Overview of cell separation techniques and strategies4.3 Stem cell-based separation methods4.4 Conclusions and future trends4.5 ReferencesChapter 5: Microscale technologies for stem cell cultureAbstract:5.1 Introduction5.2 Types of microscale technologies5.3 Microscale platforms for cell culture5.4 High-throughput and combinatorial screening applications5.5 Stem cell research5.6 Conclusions and future trends5.7 ReferencesChapter 6: Stem cells and regenerative medicineAbstract:6.1 Introduction6.2 Sources of cells for regenerative medicine6.3 Cell processing for regenerative medicine: regulatory guidelines6.4 Regenerative medicine and cellular therapy applications6.5 Conclusions and future developments6.6 ReferencesConclusions7.1 Concluding comments7.2 ReferencesIndex
