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Full Description
Genome editing offers a powerful tool to significantly accelerate crop-breeding programs in order to develop new and improved varieties. It allows precise modification of an organism's DNA sequence, often by creating targeted double-strand breaks at specific locations. The CRISPR-Cas system has emerged as the preferred method of gene editing and offers a powerful technology for crop improvement. The use of CRISPR in plant research has led to significant improvements in crop performance in terms of yield, nutrition, stress tolerance and resistance against agricultural pests and diseases. This book explores the cutting-edge field of genome editing, its applications and potential to revolutionize the genetic improvement of crops. It includes: Foundational concepts and historical context of genome editing (GE). Structure and mechanisms of various genome editing techniques. Application of GE for trait improvements in plants. Regulatory, biosafety, and ethical considerations. This is a valuable resource for researchers in crop genetic improvement, graduate and postgraduate students in molecular biology and biotechnology programs, and professionals in the field.
Contents
1: Historical Perspectives of Cutting-Edge Genome Editing Techniques 2: Classification and Nomenclature of Different Site-Specific Nucleases for Genome Editing 3: Structure and Mechanism of Zinc Finger Nucleases mediated genome editing in Plants 4: Structure and mechanism of Transcription Activator-like Effector Nuclease mediated Genome Editing in Plants 5: Structure and mechanism of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) / CRISPR Associated Nuclease 9 (Cas9)-Mediated Genome Editing in Plants 6: Multiplex Genome Editing in Plants 7: Delivering Genome Editing Reagents into the Plants: Agrobacterium and Non-Agrobacterium-Mediated Approaches 8: Recent Developments to Accelerate On-Target And Minimize Off-Target Mutations during Genome Editing in Plants 9: Estimation and Quantification Techniques to Analyse Targeted Mutations during Genome Editing 10: Application of CRISPR-Cas9 in Agriculture and Forestry 11: Application and Prospects of DNA-Free Genome Modifications in Crop Plants through Genome Editing 12: Plant Genome Editing for Nutritional Improvement 13: Genome Editing to Accelerate Biotic Stress Resistance in Crop Plants 14: Genome Editing to Improve Abiotic Stress Tolerance in Crop Plants 15: Genome Editing for Herbicide Tolerance in Crop Plants 16: Genome Editing in Improving Photosynthesis in Crop Plants 17: Plant Genome Editing to Explain Metabolic Pathways in Plants 18: Regulatory, Biosafety, and Ethical Perspectives of Plant Genome Editing
