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Full Description
This book, first of this new two-volume set, provides an informative tour of the basics of biotechnology to recent advances in biotechnology. Knowledge of new and fresh approaches is a prerequisite to solving plant biological problems, and to this end, the editors have brought together a group of contributors who address the most recent techniques and their applications in plant biotechnology. The chapters discuss some recent techniques such as TILLING (Targeting Induced Local Lesions In Genomes), advances in molecular techniques to study diversity, protein purification, and methods and analysis in protein-protein interaction detection. The volume also covers molecular markers and QTL mapping, including four chapters that deal with different molecular markers, development of mapping populations, and association mapping for dissecting the genetic basis of complex traits in plants in sufficient detail.
The knowledge of biotechnology techniques and their applications will be valuable for researchers and scientists as well as for the many students engaged in plant biotechnology studies.
Contents
History of Biotechnology. Scope and Importance of Plant Biotechnology in Crop Improvement. Scope of Plant Biotechnology in the Developing Countries. Sterilization Technique. Basic Principles and Recent Advances in Anther/Pollen Culture for Crop Improvement. Embryo Culture and Endosperm Culture. Callus Induction. Protoplast Isolation and Fusion. Somaclonal Variation. Somaclonal Variation: A Tissue Culture Approach to Crop Improvement. Restriction Endonucleases. Ligation of Insert DNA in to Cloning Vector. Blotting Techniques. Advances in PCR Technology and RNA Interference. TILLING: Genome Poking with Diligences and Constraints. Advances in Molecular Techniques to Study Diversity. Protein Purification: Science and Technology. Protein-Protein Interaction Detection: Methods and Analysis. Molecular Markers in Plant Biotechnology. Development of Mapping Populations. Principles and Practices of Mapping QTLs in Plants. Association Mapping: A Tool for Dissecting the Genetic Basis of Complex Traits in Plants.
