Full Description
In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.
Contents
General Overview of Neuronal Cell Culture.- Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology.- Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12.- Murine Teratocarcinoma-Derived Neuronal Cultures.- Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons.- Mouse Enteric Neuronal Cell Culture.- Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications.- Derivation of Neuronal Cells from Fetal Normal Human Astrocyte (NHA).- Slice Culture Modeling of Central Nervous System (CNS) Viral Infection.- Neurospheres and Glial Cell Cultures: Immunocytochemistry for Cell Phenotyping.- Transfection of Neuronal Cultures.- Lentiviral Transduction of Neuronal Cells.- Compartmentalized Neuronal Cultures.- Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates.
