Full Description
Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors focuses on direct measurement sensors, indirect methods, ligands, and related technologies, including methods involving electrochemical detectors, recognition ligands, antibodies, aptamers, and peptides, amongst many other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.
Contents
Mechanical Detectors.- A Set of Piezoelectric Biosensors Using Cholinesterases.- Piezoelectric Biosensors for Aptamer—Protein Interaction.- Piezoelectric Quartz Crystal Resonators Applied for Immunosensing and Affinity Interaction Studies.- Biosensors Based on Cantilevers.- Piezoelectric-Excited Millimeter-Sized Cantilever Biosensors.- Electrochemical Detectors.- Preparation of Screen-Printed Electrochemical Immunosensors for Estradiol, and Their Application in Biological Fluids.- Electrochemical DNA Biosensors: Protocols for Intercalator-Based Detection of Hybridization in Solution and at the Surface.- Electrochemical Biosensor Technology: Application to Pesticide Detection.- Electrochemical Detection of DNA Hybridization Using Micro and Nanoparticles.- Electrochemical Immunosensing Using Micro and Nanoparticles.- Methods for the Preparation of Electrochemical Composite Biosensors Based on Gold Nanoparticles.- Lateral Flow.- Immunochromatographic Lateral Flow Strip Tests.- Liposome-Enhanced Lateral-Flow Assays for the Sandwich-Hybridization Detection of RNA.- Rapid Prototyping of Lateral Flow Assays.- Lateral Flow Colloidal Gold-Based Immunoassay for Pesticide.- Ligands.- Synthesis of a Virus Electrode for Measurement of Prostate Specific Membrane Antigen.- In Vivo Bacteriophage Display for the Discovery of Novel Peptide-Based Tumor-Targeting Agents.- Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands.- Biosensor Detection Systems: Engineering Stable, High-Affinity Bioreceptors by Yeast Surface Display.- Antibody Affinity Optimization Using Yeast Cell Surface Display.- Using RNA Aptamers and the Proximity Ligation Assay for the Detection of Cell Surface Antigens.- In Vitro Selection of Protein-Binding DNA Aptamers as Ligandsfor Biosensing Applications.- Protein and DNA Preparation.- Immobilization of Biomolecules onto Silica and Silica-Based Surfaces for Use in Planar Array Biosensors.- Rapid DNA Amplification Using a Battery-Powered Thin-Film Resistive Thermocycler.
