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Full Description
Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. As a volume in the highly successful Methods in Molecular Biology™ series, each chapter includes an introduction to its subject matter, a list of necessary materials, step-by-step, readily reproducible laboratory protocols, and a Notes section, which provides invaluable tips and tricks to safeguard against potential mishaps and pitfalls.
Cutting-edge and comprehensive, Adipose Tissue Protocols: Second Edition is an essential manual for newcomers tothis burgeoning area as well as a valuable addition to the laboratories and offices of more experienced researchers.
Contents
Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders.- Choosing an Adipose Tissue Depot for Sampling.- Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass.- Generation of Adipose Tissue-Specific Transgenic Mouse Models.- Angiogenesis in Adipose Tissue.- Adipose Organ Nerves Revealed by Immunohistochemistry#.- Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow.- Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues.- Application of Lipidomics and Metabolomics to the Study of Adipose Tissue.- Applications of Proteomics to the Study of Adipose Tissue.- Application of DNA Microarray to the Study of Human Adipose Tissue/Cells.- Differentiation of Adipose Stem Cells.- Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells.- Explant Cultures of White Adipose Tissue.- Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue.- Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue.- Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells.- Application of RNA Interference Techniques to Adipose Cell Cultures.- RNA Isolation and Real-Time Quantitative RT-PCR.- Study of Adipose Tissue Gene Expression by In Situ Hybridization.- Application of Immunocytochemistry and Immunofluorescence Techniques to Adipose Tissue and Cell Cultures.- Determination of Lipolysis in Isolated Primary Adipocytes.- Study of Glucose Uptake in Adipose Cells.- Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction.
