Full Description
Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls.
Expansive and current, Genomics Protocols, 2nd Edition is the perfect update for researchers working in this integral, growing field.
Contents
Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield.- Scanning for DNA Variants by Denaturant Capillary Electrophoresis.- Identification of SNPs, or Mutations in Sequence Chromatograms.- BeadArray-Based Genotyping.- Microsatellite-Based Candidate Gene Linkage Analysis Studies.- Full Complexity Genomic Hybridization on 60-mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH).- Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification.- Application of Microarrays for DNA Methylation Profiling.- Genomewide Identification of Protein Binding Locations Using Chromatin Immunoprecipitation Coupled with Microarray.- Transcriptional Profiling of Small Samples in the Central Nervous System.- Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin-Embedded Tissues Using Randomly Assembled Bead Arrays.- Expression Profiling of microRNAs in Cancer Cells: Technical Considerations.- Identification of Disease Biomarkers by Profiling of Serum Proteins Using SELDI-TOF Mass Spectrometry.- The Applicability of a Cluster of Differentiation Monoclonal Antibody Microarray to the Diagnosis of Human Disease.- Protein Profiling Based on Two-Dimensional Difference Gel Electrophoresis.- Quantitative Protein Profiling by Mass Spectrometry Using Isotope-Coded Affinity Tags.- Quantitative Protein Profiling by Mass Spectrometry Using Label-Free Proteomics.- Using 2D-LC-MS/MS to Identify Francisella tularensis Peptides in Extracts from an Infected Mouse Macrophage Cell Line.- Baculovirus Expression Vector System: An Emerging Host for High-Throughput Eukaryotic Protein Expression.- Coimmunoprecipitation and Proteomic Analyses.- Tandem Affinity Purification Combined with Mass Spectrometry to Identify Components of Protein Complexes.- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo.- Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment Complementation.- Subcellular Localization of Intracellular Human Proteins by Construction of Tagged Fusion Proteins and Transient Expression in COS-7 Cells.- GeneFAS: GeneFAS: A Tool for the Prediction of Gene function Using Multiple Sources of Data.- Comparative Genomics-Based Prediction of Protein Function.- Design, Manufacture, and Assay of the Efficacy of siRNAs for Gene Silencing.
