Full Description
Distinguished international experts offer critical reviews of the major categories of posttranslational protein modification in neurons, as well as a comprehensive collection of state-of-the-art techniques for their analysis. They provide detailed and specific methods for studying protein phosphorylation/dephospohorylation, methylation, long chain fatty acylation, ADP-ribosylation, glycosylation, and glycosylphosphatidylinositol anchors. Using both conventional analytical and novel molecular biological approaches, these experts present important techniques and reagents and cite numerous examples from the neuroscience literature in a way that allows investigators to determine the best experimental approaches for their own systems. The book offers the most complete review of protein kinase and phosphatase inhibitor applications available.
Contents
Analysis of Protein Phosphorylation in Intact Cells and Extracts.- Analysis of Protein Dephosphorylation in Intact Cells and Extracts.- Protein Phosphorylation and Dephosphorylation in Isolated Nerve Terminals (Synaptosomes).- Protein Kinase and Phosphatase Inhibitors.- Phosphorylation State-Specific Antibodies.- Protein Tyrosine Phosphorylation.- Identification of Posttranslational Modification Sites by Site-Directed Mutagenesis.- Protein methylation in the nervous system.- Long-Chain Fatty Acylation of Proteins.- Protein ADP-Ribosylation.- Glycosylation and Glycosylphosphatidylinositol Membrane Anchors.
