Full Description
This book covers the broad field of cellular, molecular, preclinical, and clinical imaging either associated with or combined with photodynamic therapy (PDT). It showcases how this approach is used clinically for cancer, infections, and diseases characterized by unwanted tissue such as atherosclerosis or blindness. Because the photosensitizers are also fluorescent, the book also addresses various imaging systems such as confocal microscopy and small animal imaging systems, and highlights how they have been used to follow and optimize treatment, and to answer important mechanistic questions. Chapters also discuss how imaging has made important contributions to clinical outcomes in skin, bladder, and brain cancers, as well as in the development of theranostic agents for detection and treatment of disease. This book provides a resource for physicians and research scientists in cell biology, microscopy, optics, molecular imaging, oncology, and drug discovery.
Contents
INTRODUCTION. Looking out the optical window: Physical principles and instrumentation of imaging in photodynamic therapy. Photochemistry and photophysics of PDT and photosensitizers. IN VITRO MICROSCOPY FOR PHOTOSENSITIZER LOCALIZATION IN CELLS. Phthalocyanines in photodynamic therapy. Singlet oxygen luminescence imaging: A prospective tool in bioscience? Microbial biofilms and antimicrobial photodynamic therapy. High-content imaging for photosensitizer screening. IN VITRO MICROSCOPY OF CELL DAMAGE AND DEATH PROCESSES AFTER PDT. Enhanced efficacy of photodynamic therapy via an iron-lysosome-mitochondria connection: Studies with phthalocyanine 4. Role of cell death pathways in response to photodynamic therapy in gliomas. In search of specific PDT photosensitizers: Subcellular localization and cell death pathways. THERANOSTIC AGENTS AND NANOTECHNOLOGY. Quantum dots in PDT. Tetrapyrrole-based theranostic combinations of photodynamic action and magnetic resonance imaging. Theranostic applications of photodynamic molecular beacons. Tumor-specific imaging and photodynamic therapy targeting the urokinase receptor. SMALL ANIMAL IMAGING. Vascular imaging in photodynamic therapy. Photosensitizer activity imaging on the microscopic scale. Bioluminescence imaging for monitoring the effectiveness of photodynamic therapy for infections in animal models. CLINICAL IMAGING. Imaging of photosensitizers in skin. Brain tumor imaging with ALA. PDT of non-muscle-invasive bladder cancer with Hexylester Aminolevulinate: Optimization of the illumination wavelengths by fluorescence spectroscopy and imaging. Endoscopic imaging and photodynamic therapy. Spectroscopic imaging in prostate PDT. Fluorescent-guided resection in clinical oncology.
