Full Description
This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.
Contents
High-throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell Lines.- Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells.- High-throughput Analysis of Endocrine Disrupting Compounds using BLYES and BLYAS Bioluminescent Yeast Bioassays.- Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced ilux Operon.- Bioluminescent Imaging and Tracking of Bacterial Transport in Soils.- In vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish.- In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery.- Multiplex Imaging of Polymicrobial Communities - Murine Models to Study Oral Microbiome Interactions.- Bioluminescence Imaging to Study Mature Biofilm Formation by Candida spp. and Antifungal Activity in vitro and in vivo.- Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson's Disease.- Continual Conscious Bioluminescent Imaging in Freely Moving Mice.- In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent Model.- Continuous and Real-time In Vivo Autobioluminescent Imaging in a Mouse Model.- In vivo Tracking of Tumor-derived Bioluminescent Extracellular Vesicles in Mice.- Bioluminescence Imaging in the Chick Chorioallantoic Membrane Assay.- Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Models.- The Application of Single Cell Bioluminescent Imaging to Monitor Circadian Rhythms of Individual Plant Cells.
