Full Description
This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.
Contents
What a Difference 30 years Makes! A Perspective on Changes in research Methodologies used to Study Toxoplasma gondii.- ToxoDB: Functional Genomics Resource for Toxoplasma and Related Organisms.- Isolation and Genotyping of Toxoplasma gondii Strains.- Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of their Sporozoites for Downstream Manipulation.- Assay for Monitoring Toxoplasma gondii Infectivity in the Laboratory Mouse.- PCR Screening of Toxoplasma gondii Single Clones Directly from 96-Well Plantes without DNA Purification.- CRISPR/Cas9-Mediated Generation of Tetracycline Repressor-Based Inducible Knock-Down in Toxoplasma gondii.- Assessing Rhyptry Secretion in T. gondii.- High-Throughput Measurement of Microneme Secretion in Toxoplasma gondii.- Plate-Based Quantification of Stimulated Toxoplasma egress.- Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondii.- Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging.- Experimental Approaches for Examining Apicoplast Biology.- Measuring Solute Transport in Toxoplasma gondii Parasites.- Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In Vivo.- Three-Dimensional Reconstruction of Toxoplasma-Neuron Interactions In Situ.- Model Systems for Studying Mechanisms of Ocular Toxoplasmosis.- Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii.- Assays to Evaluate Toxoplasma-Macrophage Interactions.- Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells.- Image-based Quantitation of Host Cell-Toxoplasma gondii Interplay using HRMan; A Host Response to Microbe Analysis Pipeline.- Metabolomic Analysis of Toxoplasma gondii Tachyzoites.- Label-Based Mass Spectrometry Approaches for Robust Quantification of the Phospho- and Total Proteome in Toxoplasma gondii.
