Full Description
This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Diabetic Nephropathy: Methods and Protocols seeks to foster new research directions and inspire ideas to enhance our understanding of diabetic nephropathy and todevelop treatments for this condition.
Contents
Diabetic Nephropathy: An Overview.- Modeling Podocyte Biology Using Drosophila Nephrocytes.- A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros.- Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic Rodent Model.- Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy.- Histological Examination of the Diabetic Kidney.- Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy.- Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy.- Transcutaneous Measurement of Glomerular Filtration Rate in Rodents.- An In Vitro Method to Analyze Glucose Uptake in Podocytes.- Glomerular Endothelial Cells: Assessment of Barrier Properties In Vitro.- Methods to Detect Endoplasmic Reticulum Stress and Apoptosis in Diabetic Nephropathy.- Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease.- Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy.- Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches.- Genetic Strategies to Understand Human Diabetic Nephropathy: In Silico Strategies for Molecular Data - Association Studies.- Assessment of Urinary MicroRNAs by Quantitative Polymerase Chain Reaction in Diabetic Nephropathy Patients.- Metabolomic and Proteomic Techniques for Establishing Biomarkers and Improving Our Understanding of Pathophysiology in Diabetic Nephropathy.- Formation of Mature Nephrons by Implantation of Human Pluripotent Stem Cell-Derived Progenitors into Mice.- Generating Mutant Renal Cell Lines Using CRISPR Technologies.
